The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality

BMC Veterinary Research
Jane-Fang YuPei-Shiue Tsai

Abstract

Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the semen plug and accelerate the semen liquefaction process in zoo captive chimpanzee species (Pan troglodytes). We showed that incubation of chimpanzee ejaculates with 0.1% type I collagenase efficiently and significantly (p < 0.05) releases 2.7-fold more spermatozoa from the coagulated ejaculates, and this degelification process did not alter sperm morphology or viability; nor did it stimulate spontaneous capacitation or an acrosome reaction as assessed by tyrosine phosphorylation and peanut agglutinin stains; moreover, based on computer assisted sperm analysis assay, motility-related parameters remained similar to those of untreated spermatozoa. When collagenase effects were evaluated on cryopreserved sperm samples, we observed post collagenase treatment in which 2.5% glycerol, as a cryoprotectant, preserved sperm acrosome integrity better than 7.8%; ho...Continue Reading

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Citations

Oct 24, 2019·Animals : an Open Access Journal From MDPI·Eliana R SteinbergMarta D Mudry
Dec 10, 2020·Animals : an Open Access Journal From MDPI·Yu-Hsin ChenPei-Shiue Jason Tsai
Sep 15, 2020·Molecular and Cellular Endocrinology·Guillaume Martinez, Cécile Garcia

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

UltiMate
Cellsens
CASA
ImageJ

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