The efficacy of HBOC-201 in ex situ gradual rewarming kidney perfusion in a rat model

Artificial Organs
Paria MahboubKorkut Uygun

Abstract

Gradual rewarming from hypothermic to normothermic is a novel perfusion modality with superior outcome to sudden rewarming to normothermic. However, the identification of an oxygen carrier that could function at a temperature range from 4 to 7°C or whether it is necessary to use oxygen carrier during kidney rewarming, remains unresolved. This study was designed to test the use of a hemoglobin-based oxygen carrier (HBOC) during gradual kidney rewarming as an alternative to simple dissolved oxygen. In this study, 10 rat kidneys were randomly divided into the control and the HBOC group. In the control group, no oxygen carrier was used during rewarming perfusion and the perfusion solution was oxygenated only by applying diffused carbogen flow. The protocol mimicked a donor after circulatory death (DCD) kidney transplantation, where after 30 minutes warm ischemia and 120 minutes cold storage in University of Wisconsin solution, the DCD kidneys underwent gradual rewarming from 10 to 37°C during 90 minutes with or without HBOC. This was followed by 30 minutes of warm ischemia in room temperature to mimic the anastomosis time and 120 minutes of reperfusion at 37°C to mimic the early post-transplant state of the graft. The HBOC group de...Continue Reading

References

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Citations

Aug 12, 2020·Artificial Organs·Jan CzogallaTobias B Huber
Dec 18, 2019·Artificial Organs·Majid RezaeiBahar Bassiri Gharb
Nov 5, 2020·International Journal of Molecular Sciences·Sebastien GiraudThierry Hauet

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biopsies

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SPSS

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