Abstract
Several rich sources of polyphenols stimulate the endothelial formation of nitric oxide (NO), a potent vasoprotecting factor, via the redox-sensitive activation of the PI3-kinase/Akt pathway leading to the phosphorylation of endothelial NO synthase (eNOS). The present study examined the molecular mechanism underlying the stimulatory effect of epicatechins on eNOS. NO-mediated relaxation was assessed using porcine coronary artery rings in the presence of indomethacin, and charybdotoxin plus apamin, inhibitors of cyclooxygenases and EDHF-mediated responses, respectively. The phosphorylation level of Akt and eNOS was assessed in cultured coronary artery endothelial cells by Western blot, and ROS formation using dihydroethidine. (-)-Epigallocatechin-3-O-gallate (EGCg) caused endothelium-dependent relaxations in coronary artery rings and the phosphorylation of Akt and eNOS in endothelial cells. These responses were inhibited by membrane-permeant analogues of superoxide dismutase and catalase, whereas native superoxide dismutase, catalase and inhibitors of major enzymatic sources of reactive oxygen species including NADPH oxidase, xanthine oxidase, cytochrome P450 and the mitochondrial respiration chain were without effect. The EGCg ...Continue Reading
References
Jul 2, 1998·Circulation Research·F J MillerB L Davidson
Sep 8, 1999·Circulation·J H SteinJ D Folts
Aug 30, 2000·Annals of Epidemiology·S SasazukiA Takeshita
Nov 10, 2000·Biochemical and Biophysical Research Communications·V Ullrich, M Bachschmid
Feb 21, 2002·Biochimica Et Biophysica Acta·Manabu MochizukiTokuji Ikeda
Dec 3, 2003·The Journal of Biological Chemistry·Mario LorenzVerena Stangl
Jun 11, 2004·Journal of the American College of Nutrition·Mary B EnglerMichele L Mietus-Snyder
Jul 14, 2004·British Journal of Pharmacology·Mamadou NdiayeValérie B Schini-Kerth
Oct 7, 2004·European Journal of Pharmacology·Jean-Claude StocletValérie B Schini-Kerth
Dec 9, 2004·Coronary Artery Disease·Kalliopi KaratziMyron Mavrikakis
Dec 27, 2005·Bioscience, Biotechnology, and Biochemistry·Makoto KobayashiIkuo Ikeda
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hagen SchroeterMalte Kelm
Apr 4, 2006·Journal of the American College of Nutrition·Carmen CabreraRafael Giménez
May 16, 2006·Vascular Pharmacology·N KavantzasD Perrea
Sep 12, 2006·Cardiovascular Research·Eric AnselmValérie B Schini-Kerth
Sep 14, 2006·JAMA : the Journal of the American Medical Association·Shinichi KuriyamaIchiro Tsuji
Mar 17, 2007·The Journal of Biological Chemistry·Jeong-A KimMichael J Quon
May 17, 2007·IUBMB Life·Cesar G Fraga
Jul 3, 2007·Free Radical Biology & Medicine·Shengmin SangChung S Yang
Oct 12, 2007·Clinical Science·Indika EdirisingheC Tissa Kappagoda
Jan 22, 2009·Journal of Vascular Research·Socorro Vanesca Frota MadeiraValérie B Schini-Kerth
Feb 28, 2009·Journal of Cardiovascular Pharmacology·Eric AnselmValérie B Schini-Kerth
Jun 13, 2009·Vascular Pharmacology·Modou Oumy KaneValérie B Schini-Kerth
Aug 6, 2007·Tetrahedron·Kumi OsanaiTak Hang Chan
Citations
May 3, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Jung-Ok LeeValérie B Schini-Kerth
Feb 11, 2012·European Journal of Nutrition·Annalisa AggioClaudio Ferri
Sep 17, 2013·Journal of Agricultural and Food Chemistry·Aurica M KaufeldHerbert Kolodziej
Jul 23, 2011·Food & Function·Cyril AugerValérie B Schini-Kerth
Aug 12, 2010·Antioxidants & Redox Signaling·Gabriela M KusterOtmar Pfister
Feb 25, 2011·The Journal of Clinical Endocrinology and Metabolism·Stefano RizzaMichael J Quon
Feb 27, 2013·PloS One·Alice TonioloChiara Bolego
Aug 11, 2011·European Journal of Pharmacology·Kazuo ObaraTomohisa Ishikawa
Sep 13, 2016·Journal of Food Biochemistry·Claudia Elena VarelaIsrael Ramirez-Sanchez
Aug 27, 2013·Nitric Oxide : Biology and Chemistry·Jong Hun KimValérie B Schini-Kerth
Dec 4, 2014·Nutrition·Kazuo YamagataYukio Yamori
Jul 11, 2014·Journal of Medicinal Food·Cyril AugerValérie B Schini-Kerth
Aug 19, 2014·PloS One·Faraj ZgheelValérie B Schini-Kerth
Nov 13, 2016·The Journal of Nutritional Biochemistry·Huei L H NgMichelle A Keske
Dec 18, 2015·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sonia Khemais-BenkhiatValérie B Schini-Kerth
Dec 12, 2017·Journal of Nutritional Science and Vitaminology·Sachiko NomuraAtsushi Nesumi
Aug 6, 2011·Journal of Muscle Research and Cell Motility·M E KargacinGary J Kargacin
Oct 30, 2012·Food & Function·Ikuko KuritaValérie B Schini-Kerth
Feb 9, 2013·Bioscience, Biotechnology, and Biochemistry·Chul Young KimMin-Ho Oak
Feb 26, 2015·Journal of Cardiovascular Pharmacology·Mario LorenzVerena Stangl
Mar 15, 2016·Journal of Cardiovascular Pharmacology·Cyril AugerValérie B Schini-Kerth
Jan 3, 2020·Journal of Cardiovascular Pharmacology·Kazuo Yamagata
Sep 8, 2020·Phytotherapy Research : PTR·Mahdieh Sadat MohsenzadehHossein Hosseinzadeh
May 20, 2017·Journal of Cellular and Molecular Medicine·Guillaume KreutterFlorence Toti
May 13, 2020·Antioxidants·Mario LorenzVerena Stangl
May 4, 2021·Transplantation Proceedings·Mohamad KassemFlorence Toti
Jun 25, 2014·Journal of Natural Products·Aurica M KaufeldHerbert Kolodziej
Sep 24, 2021·Critical Reviews in Food Science and Nutrition·Mamali DasSeyed Mohammad Nabavi