The EGCg-induced redox-sensitive activation of endothelial nitric oxide synthase and relaxation are critically dependent on hydroxyl moieties

Biochemical and Biophysical Research Communications
Cyril AugerValérie B Schini-Kerth

Abstract

Several rich sources of polyphenols stimulate the endothelial formation of nitric oxide (NO), a potent vasoprotecting factor, via the redox-sensitive activation of the PI3-kinase/Akt pathway leading to the phosphorylation of endothelial NO synthase (eNOS). The present study examined the molecular mechanism underlying the stimulatory effect of epicatechins on eNOS. NO-mediated relaxation was assessed using porcine coronary artery rings in the presence of indomethacin, and charybdotoxin plus apamin, inhibitors of cyclooxygenases and EDHF-mediated responses, respectively. The phosphorylation level of Akt and eNOS was assessed in cultured coronary artery endothelial cells by Western blot, and ROS formation using dihydroethidine. (-)-Epigallocatechin-3-O-gallate (EGCg) caused endothelium-dependent relaxations in coronary artery rings and the phosphorylation of Akt and eNOS in endothelial cells. These responses were inhibited by membrane-permeant analogues of superoxide dismutase and catalase, whereas native superoxide dismutase, catalase and inhibitors of major enzymatic sources of reactive oxygen species including NADPH oxidase, xanthine oxidase, cytochrome P450 and the mitochondrial respiration chain were without effect. The EGCg ...Continue Reading

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Citations

Aug 12, 2010·Antioxidants & Redox Signaling·Gabriela M KusterOtmar Pfister
Sep 13, 2016·Journal of Food Biochemistry·Claudia Elena VarelaIsrael Ramirez-Sanchez
Nov 13, 2016·The Journal of Nutritional Biochemistry·Huei L H NgMichelle A Keske
Dec 18, 2015·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sonia Khemais-BenkhiatValérie B Schini-Kerth
Aug 6, 2011·Journal of Muscle Research and Cell Motility·M E KargacinGary J Kargacin
Feb 9, 2013·Bioscience, Biotechnology, and Biochemistry·Chul Young KimMin-Ho Oak
Mar 15, 2016·Journal of Cardiovascular Pharmacology·Cyril AugerValérie B Schini-Kerth
Jan 3, 2020·Journal of Cardiovascular Pharmacology·Kazuo Yamagata
May 4, 2021·Transplantation Proceedings·Mohamad KassemFlorence Toti
Sep 24, 2021·Critical Reviews in Food Science and Nutrition·Mamali DasSeyed Mohammad Nabavi

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