PMID: 8970966Dec 1, 1996Paper

The eIF4G-eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase

Journal of Virology
A HaghighatN Sonenberg

Abstract

The 2A proteinases (2Apro) of certain picornaviruses induce the cleavage of the eIF4G subunit of the cap-binding protein complex, eIF4F. Several reports have demonstrated that 2Apro of rhinovirus and coxsackievirus B4 cleave eIF4G directly. However, it was suggested that in poliovirus infection, the 2Apro induces the activation of a cellular proteinase which in turn cleaves eIF4G. Furthermore, it is not clear whether eIF4G is cleaved as part of the eIF4F complex or as an individual polypeptide. To address these issues, recombinant eIF4G was purified from Sf9 insect cells and tested for cleavage by purified rhinovirus 2Apro. Here we report that eIF4G alone is a relatively poor substrate for cleavage by the rhinovirus 2Apro. However, an eIF4G-eIF4E complex is cleaved efficiently by the 2Apro, suggesting that eIF4F is a preferred substrate for cleavage by rhinovirus 2Apro. Furthermore, 2Apr drastically reduced the translation of a capped mRNA. An eIF4G-eIF4E complex, but not eIF4G alone, was required to restore translation.

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M J HewlettD Baltimore
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A NomotoE Wimmer
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N SonenbergA J Shatkin
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J Hambidge, P Sarnow
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E E WyckoffE Ehrenfeld
Mar 1, 1990·Molecular and Cellular Biology·F RozenN Sonenberg
Nov 1, 1985·Molecular and Cellular Biology·H D BernsteinD Baltimore
Oct 24, 1995·Biochemical and Biophysical Research Communications·R AldabeL Carrasco
Sep 1, 1996·Microbiological Reviews·G J Belsham, N Sonenberg

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Citations

Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M GaleM G Katze
Jul 4, 2001·Journal of Virology·H Hahn, A C Palmenberg
Apr 16, 2005·The Journal of General Virology·Luiza DeszczJoachim Seipelt
Oct 31, 2008·The EMBO Journal·Mohammad A Mir, Antonito T Panganiban
Nov 6, 2009·Journal of Virology·Julianne L GarreyEric Jan
Sep 20, 2015·Protein Science : a Publication of the Protein Society·Martina AumayrTim Skern
Oct 4, 2016·FEMS Yeast Research·Satarupa Das, Biswadip Das
Feb 10, 2017·Journal of Virology·Kelly WattersAnn C Palmenberg
Jan 7, 1998·Molecular and Cellular Biology·A GradiN Sonenberg
Sep 14, 2002·The Journal of Biological Chemistry·Nicole FoegerTim Skern
Feb 28, 2004·FEBS Letters·Luiza DeszczErnst Kuechler
Jun 7, 2003·The Journal of Biological Chemistry·Nicole FoegerTim Skern
May 5, 2011·Journal of Biomedicine & Biotechnology·Alfredo CastellóLuis Carrasco
Apr 2, 2010·Journal of Virology·Holly A SaffranJames R Smiley
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kateryna FeoktistovaChristopher S Fraser
Apr 3, 2013·Molecular and Cellular Biology·Marie-Josée FournierRachid Mazroui
Dec 24, 2015·Viruses·Prabuddha S PathinayakePeter A B Wark
May 19, 2017·Journal of Virology·Subbiah JeevaMohammad A Mir
Jan 9, 2020·Journal of Biomedical Science·Ming-Chih LaiRobert Y L Wang
Aug 29, 1997·The Journal of Biological Chemistry·A Haghighat, N Sonenberg
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D S Lyles
Jan 7, 2009·Molecular and Cellular Biology·Keith A SpriggsAnne E Willis

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