The electric dipole moment of rhodopsin solubilized in Triton X-100

Biophysical Journal
D C Petersen, R A Cone

Abstract

The electric dipole moment of solubilized rhodopsin was determined with dielectric dispersion measurements. Rhodopsin was extracted from disc membranes of cattle rod outer segments with the nonionic detergent Triton X-100. The dipole moment of rhodopsin at its isoionic point in the detergent micelle is 720 D (150 charge-A). This value is comparable to dipole moments of nonmembrane proteins, especially those which tend to aggregate or polymerize. Flash irradiation of the rhodopsin results in an increase in the dipole moment of about 25 D (5 charge-A). The light-induced increase in dipole moment appears to be composed of two parts--a faster component related to a change in the number of protons bound by rhodopsin and a slower component apparently independent of the change in proton binding.

Citations

Apr 21, 1977·Biophysics of Structure and Mechanism·A K Wright
Jul 1, 1977·Photochemistry and Photobiology·W L Stone, E A Dratz
Aug 20, 1979·Biochimica Et Biophysica Acta·M Montal

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