The electroneutral Na⁺:HCO₃⁻ cotransporter NBCn1 is a major pHi regulator in murine duodenum.

The Journal of Physiology
Mingmin ChenUrsula Seidler

Abstract

Duodenocyte pHi control and HCO3 − secretion protects the proximal duodenum against damage by gastric acid. The molecular details of duodenocyte pH control are not well understood. A selective duodenal expression (within the upper GI tract) has been reported for the electroneutral Na+:HCO3 − cotransporter NBCn1 (Slc4a7). We aimed to determine the role of NBCn1 and NBCe2 in duodenocyte intracellular pH regulation as well as basal and agonist-stimulated duodenal bicarbonate secretion (JHCO3 −), exploiting mouse models of genetic slc4a7 and slc4a5 disruption. Basal and forskolin (FSK)-stimulated JHCO3 − was measured by single-pass perfusion in the duodenum of slc4a7−/− and slc4a7+/+ as well as slc4a5−/− and slc4a5+/+ mice in vivo, and by pH-stat titration in isolated duodenal mucosa in vitro. Duodenocyte HCO3 − uptake rates were fluorometrically assessed after acidification of intact villi and of isolated duodenocytes. Slc4a7−/− mice displayed significantly lower basal and FSK-stimulated duodenal HCO3 − secretion than slc4a7+/+ littermates in vivo. FSK-stimulated HCO3 − secretion was significantly reduced in slc4a7−/− isolated duodenal mucosa. Na+- and HCO3 −-dependent base uptake rates were significantly decreased in slc4a7−/− co...Continue Reading

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Citations

Dec 10, 2015·Frontiers in Physiology·Ying LiuLi-Ming Chen
Jun 27, 2014·Pflügers Archiv : European journal of physiology·Xuemei LiuUrsula Seidler
May 28, 2016·Pflügers Archiv : European journal of physiology·Qin YuUrsula Seidler
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
Aug 11, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Susrutha Puthanmadhom NarayananAdil E Bharucha
Feb 1, 2018·American Journal of Physiology. Cell Physiology·Mark D Parker
Dec 16, 2020·Behavioural Brain Research·Inyeong ChoiEbbe Boedtkjer

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