PMID: 6968711May 1, 1980Paper

The electrophoretic mobility of alpha 1-antitrypsin in sputum and its relationship to protease inhibitory capacity, leucocyte elastase concentrations and acute respiratory infection

Hoppe-Seyler's Zeitschrift für physiologische Chemie
D Burnett, R A Stockley

Abstract

The electrophoretic mobility of uncomplexed alpha 1-antitrypsin was studied in the sol phase of sputum from 14 patients with chronic bronchitis. The mobility of alpha 1-antitrypsin in the sputum of each subject was reduced compared to the mobility in serum (mean sputum value = 71.1% of albumin mobility; mean serum value = 78.5% of albumin mobility). This reduction in alpha 1-antitrypsin mobility was most significant in the sputum samples of patients with chest infections which contained free elastolytic activity (mean value in cases where no enzyme activity was found = 74.9%; in cases with enzyme activity = 66.1%, 2P < 0.01). There was a significant inverse relationship between alpha 1-antitrypsin mobility in sputum and concentration of immunoreactive leucocyte elastase (r = -0.739, 2P < 0.01). The addition of trypsin to the sputum samples showed failure to inhibit the enzyme despite the apparant presence of sufficient uncomplexed alpha 1-antitrypsin in these samples, suggesting this alpha 1-antitrypsin was non-functional. Proteolytic enzymes can inactivate alpha 1-antitrypsin with a reduction in its electrophoretic mobility. The results of this study suggest that the alpha 1-antitrypsin in sputum may be inactivated by lysosoma...Continue Reading

Citations

Jan 28, 2006·American Journal of Respiratory and Critical Care Medicine·David A Lomas
Jun 27, 2006·American Journal of Respiratory and Critical Care Medicine·John R HurstJadwiga A Wedzicha
Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Robert A StockleyLee J Dowson
Jun 1, 1986·The Journal of Clinical Investigation·P J OssannaS J Weiss
Jan 1, 1991·Annals of the New York Academy of Sciences·R A MumfordK Hand
Oct 4, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Anna Linja-ahoVuokko L Kinnula
Apr 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R A StockleyD Brunett

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