The electrophysiological development of cardiomyocytes

Advanced Drug Delivery Reviews
Jie LiuPeter H Backx

Abstract

The generation of human cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs) has become an important resource for modeling human cardiac disease and for drug screening, and also holds significant potential for cardiac regeneration. Many challenges remain to be overcome however, before innovation in this field can translate into a change in the morbidity and mortality associated with heart disease. Of particular importance for the future application of this technology is an improved understanding of the electrophysiologic characteristics of CMs, so that better protocols can be developed and optimized for generating hPSC-CMs. Many different cell culture protocols are currently utilized to generate CMs from hPSCs and all appear to yield relatively “developmentally” immature CMs with highly heterogeneous electrical properties. These hPSC-CMs are characterized by spontaneous beating at highly variable rates with a broad range of depolarization-repolarization patterns, suggestive of mixed populations containing atrial, ventricular and nodal cells. Many recent studies have attempted to introduce approaches to promote maturation and to create cells with specific functional properties. In this review, we summarize the studies ...Continue Reading

References

Sep 1, 1992·Circulation Research·Y SakakibaraD H Singer
Mar 1, 1992·The Journal of General Physiology·A C Zygmunt, W R Gibbons
Jan 1, 1988·Circulation Research·S H Litovsky, C Antzelevitch
Nov 1, 1988·The Journal of Physiology·W R Giles, Y Imaizumi
Oct 1, 1973·Biochemical and Biophysical Research Communications·S MartinG S Levey
Jan 1, 1969·Circulation Research·J R CouchH E Hoff
Mar 1, 1982·Pflügers Archiv : European journal of physiology·S KokubunH Irisawa
Oct 1, 1982·Cardiovascular Research·L ArnoldD A Eisner
Apr 1, 1981·Japanese Circulation Journal·Y Watanabe
Jan 1, 1993·Annual Review of Physiology·D DiFrancesco
Aug 1, 1994·Pflügers Archiv : European journal of physiology·M SchneiderR Rüdel
Jan 1, 1996·Circulation Research·M P DaviesR S Kass
Jan 1, 1995·Reproduction, Fertility, and Development·J J Smolich
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Jul 1, 1996·Circulation Research·L WangH J Duff
Jun 17, 1998·The Journal of Physiology·B LondonP B Bennett
Oct 13, 1998·Journal of the American College of Cardiology·E DrouinF Charpentier
Nov 7, 1999·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·M C LeivaJ C Huhta
Mar 23, 2000·Cardiovascular Research·Y QuM Boutjdir
Mar 29, 2000·The Journal of Biological Chemistry·Y HashimotoT Yanagisawa

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Citations

Jun 11, 2016·Journal of Pharmacological and Toxicological Methods·Thomas ColatskyNorman Stockbridge
Jun 3, 2017·European Heart Journal·Francisco Fernández-AvilésUNKNOWN Delivery, Navigation, Tracking and Assessment Subcommittee
Aug 11, 2017·Stem Cells and Development·Maj Linea VestergaardClaus Yding Andersen
Apr 13, 2018·Cardiovascular Research·Scott BarichelloZachary Laksman
Mar 29, 2020·The Journal of Pharmacology and Experimental Therapeutics·Weiwei JiangYin Wang
Jun 10, 2020·Cardiovascular Research·Giovanni Maroli, Thomas Braun
Mar 25, 2017·Frontiers in Cell and Developmental Biology·Xuetao Sun, Sara S Nunes
May 23, 2017·Frontiers in Cell and Developmental Biology·Gaetano J Scuderi, Jonathan Butcher
Jul 15, 2017·Scientific Reports·Zachary LaksmanPeter H Backx
Sep 5, 2018·BMC Complementary and Alternative Medicine·Zhiguo ZhangYanjing Chen
Dec 13, 2016·Nature Biotechnology·Stephanie I ProtzeGordon M Keller
Feb 6, 2020·Nature Reviews. Cardiology·Elaheh KarbassiCharles E Murry
Apr 17, 2020·Frontiers in Cardiovascular Medicine·Pim R R van GorpAntoine A F de Vries
Aug 6, 2020·International Journal of Molecular Sciences·Emre BektikDa-Zhi Wang
Oct 31, 2018·International Journal of Molecular Sciences·Brian X WangCesare M N Terracciano
Dec 2, 2020·International Journal of Molecular Sciences·Junjun LiYoshiki Sawa
Dec 17, 2020·Journal of Cardiovascular Pharmacology·Michelangelo PaciJussi T Koivumäki
Mar 18, 2021·Cardiovascular Research·Valentina PaloschiLars Maegdefessel
May 4, 2021·Frontiers in Cell and Developmental Biology·Danielle PretoriusJianyi Zhang
Oct 30, 2021·International Journal of Stem Cells·Yingnan LiaoYan Wang

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