The elementary events underlying force generation in neuronal lamellipodia.

Scientific Reports
Ladan AminVincent Torre

Abstract

We have used optical tweezers to identify the elementary events underlying force generation in neuronal lamellipodia. When an optically trapped bead seals on the lamellipodium membrane, Brownian fluctuations decrease revealing the underlying elementary events. The distribution of bead velocities has long tails with frequent large positive and negative values associated to forward and backward jumps occurring in 0.1-0.2 ms with varying amplitudes up to 20 nm. Jump frequency and amplitude are reduced when actin turnover is slowed down by the addition of 25 nM Jasplakinolide. When myosin II is inhibited by the addition of 20 μM Blebbistatin, jump frequency is reduced but to a lesser extent than by Jasplainolide. These jumps constitute the elementary events underlying force generation.

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Citations

Aug 31, 2013·PloS One·Ladan AminVincent Torre
Jan 22, 2013·Biophysical Journal·Matthew B SmithDimitrios Vavylonis
Jan 20, 2015·Scientific Reports·Wasim A SayyadVincent Torre
May 8, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·David Lepzelter, Muhammad H Zaman
May 24, 2013·International Journal of Molecular Sciences·Francesco DifatoDan Cojoc
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Isaac C D LentonItia A Favre-Bulle

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Methods Mentioned

BETA
optical
optical tweezers
optical tweezer

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