The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses

PloS One
Tassanee LerksuthiratTheerapong Krajaejun

Abstract

Pythium insidiosum is a unique oomycete that can infect humans and animals. Patients with a P. insidiosum infection (pythiosis) have high rates of morbidity and mortality. The pathogen resists conventional antifungal drugs. Information on the biology and pathogenesis of P. insidiosum is limited. Many pathogens secrete proteins, known as effectors, which can affect the host response and promote the infection process. Elicitins are secretory proteins and are found only in the oomycetes, primarily in Phytophthora and Pythium species. In plant-pathogenic oomycetes, elicitins function as pathogen-associated molecular pattern molecules, sterol carriers, and plant defense stimulators. Recently, we reported a number of elicitin-encoding genes from the P. insidiosum transcriptome. The function of elicitins during human infections is unknown. One of the P. insidiosum elicitin-encoding genes, ELI025, is highly expressed and up-regulated at body temperature. This study aims to characterize the biochemical, immunological, and genetic properties of the elicitin protein, ELI025. A 12.4-kDa recombinant ELI025 protein (rELI025) was expressed in Escherichia coli. Rabbit anti-rELI025 antibodies reacted strongly with the native ELI025 in P. insidi...Continue Reading

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Citations

Aug 10, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Tassanee LerksuthiratTheerapong Krajaejun
Jan 1, 2016·Journal of Clinical Microbiology·Ruchuros InkomlueTheerapong Krajaejun
Jan 25, 2017·Antimicrobial Agents and Chemotherapy·Tassanee LerksuthiratTheerapong Krajaejun
Jan 27, 2018·Medical Mycology·Jéssica Luana ChechiSandra de Moraes Gimenes Bosco
Mar 3, 2020·PeerJ·Maria Nina ChitasombatTheerapong Krajaejun
Jun 3, 2021·Journal of Fungi·Jéssica Luana ChechiSandra de Moraes Gimenes Bosco

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Datasets Mentioned

BETA
AB971191
AB971192
AB971193
HS975204
FX528334

Methods Mentioned

BETA
PCR
glycosylation
electrophoresis

Software Mentioned

Image Lab
TBLASTN
NetOGlyc
GelQuant
BLAST
ClustalX
NetNGlyc
SignalIP
big
MASCOT

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