PMID: 8961355Nov 1, 1996Paper

The elusive role of the N-terminal extension of beta A3- and beta A1-crystallin

Protein Engineering
P J WertenW W De Jong

Abstract

beta-Crystallins are structural lens proteins with a conserved two-domain structure and variable N- and C-terminal extensions. These extensions are assumed to be involved in quaternary interactions within the beta-crystallin oligomers or with other lens proteins. Therefore, the production of beta A3- and beta A1-crystallin from the single beta A3/A1 mRNA by dual translation initiation is of interest. These crystallins are identical, except that beta A1 has a much shorter N-terminal extension that beta A3. This rare mechanism has been conserved for over 250 million years during the evolution of the beta A3/A1 gene, suggesting that the generation of different N-terminal extensions confers a selective advantage. We therefore compared the stability and association behaviour of recombinant beta A3- and beta A1-crystallin. Both proteins are equally stable in urea- and pH-induced denaturation experiments. Gel filtration and analytical ultracentrifugation established that beta A3 and beta A1 both form homodimers. In the water-soluble proteins of bovine lens, beta A3 and beta A1 are present in the same molecular weight fractions, indicating that they oligomerize equally with other beta-crystallins. 1H-NMR spectroscopy showed that residu...Continue Reading

Citations

Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Mar 13, 2014·Progress in Biophysics and Molecular Biology·Kirsten J LampiLarry L David
Jan 22, 2003·Biochemical and Biophysical Research Communications·O P Srivastava, K Srivastava
Sep 6, 2003·Experimental Eye Research·O A BatemanC Slingsby
Jul 3, 1998·International Journal of Biological Macromolecules·J A Carver, R A Lindner
Jul 17, 1999·Biochimica Et Biophysica Acta·P J WertenW W de Jong
Apr 27, 1999·Progress in Retinal and Eye Research·J A Carver
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P J WertenW W de Jong
Dec 25, 2003·The British Journal of Ophthalmology·K P BurdonM M Sale
Sep 1, 2004·Experimental Eye Research·J F HejtmancikY V Sergeev
Jun 14, 2014·Molecular Medicine Reports·Jing ZhangQinying Cao
Dec 19, 2001·Experimental Eye Research·Y UedaL L David

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