PMID: 37275Jan 1, 1979

The elution of antibodies from viable murine tumor cells

Journal of Immunological Methods
R Ehrlich, I P Witz

Abstract

Several low pH buffers were used in order to dissociate radioiodinated antibodies from sensitized living tumor cells. Three buffers were further tested for their dissociation abilities under different conditions of time and temperature, and for their influence on the eluted antibodies. The cytotoxicity mediated by these low pH buffers was also determined by viability assays. Optimal results were obtained with 0.1 M citrate buffer at pH 3.5.

References

Apr 1, 1994·Veterinary Immunology and Immunopathology·S DandapatG C Mohanty
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V DuprezA Dautry-Varsat
Jun 8, 2007·Cancer Letters·Yelena V Kovtun, Victor S Goldmacher
Apr 21, 2012·Avian Pathology : Journal of the W.V.P.A·Hermann MüllerM Rafiqul Islam
Nov 1, 1979·Arthritis and Rheumatism·A JurjusI P Witz

Related Concepts

Buffers
Tumor Cells, Uncertain Whether Benign or Malignant
Mice, Inbred BALB C
Citrate buffer
Murine
Ampholytes
Cytotoxicity
Antibody Dissociation
Observation Method - Cytotoxicity
PH.3

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