The emergence of blaOXA-48 and blaNDM among ESBL-producing Klebsiella pneumoniae in clinical isolates of a tertiary hospital in Iran

Acta Microbiologica Et Immunologica Hungarica
Mehdi MoghadampourJamshid Faghri

Abstract

The aim of this study was to investigate the prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and the most common types of carbapenemases, metallo-beta-lactamases (MBLs), and extended-spectrum beta-lactamases (ESBLs) among CRKP isolates in a tertiary hospital in Isfahan, Iran. Eighty non-repetitive clinical isolates of K. pneumoniae were obtained from different clinical specimens. Antibiotic resistance pattern of isolates was determined by disk diffusion method and production of carbapenemases and MBLs was confirmed using modified Hodge test and E-test, respectively. Molecular detection of the antibiotic resistance genes was performed using PCR. Fifty-one (63.8%) isolates have decreased susceptibility to carbapenems, of which 46 (90.2%) isolates were as carbapenemase producer and four (7.8%) isolates were positive for MBLs, phenotypically. The results of PCR showed that the prevalence of blaOXA-48, blaNDM, blaSHV, blaCTX-M, and blaTEM genes among CRKP isolates were 90.2%, 15.7%, 98%, 96.1%, and 90.2%, respectively. No isolates carrying the blaKPC, blaGES, blaIMI, blaVIM, and blaIMP genes were detected. This study showed that the production of OXA-48 is one of the main mechanisms of resistance to carbapenems in CR...Continue Reading

References

Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Jun 27, 2008·International Journal of Food Microbiology·Yin LiuXitai Huang
Jul 11, 2008·Journal of Clinical Microbiology·Musa HindiyehNathan Keller
May 26, 2011·Expert Review of Anti-infective Therapy·Gopi Patel, Robert A Bonomo
Jul 5, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D Ben-DavidG Rahav
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Dec 23, 2011·The Journal of Antimicrobial Chemotherapy·Amos AdlerYehuda Carmeli
Apr 14, 2012·The Journal of Antimicrobial Chemotherapy·Laurent PoirelPatrice Nordmann
Apr 18, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M AkovaY Carmeli
Apr 18, 2014·The Journal of Antimicrobial Chemotherapy·Evangelia VoulgariAthanassios Tsakris
Jun 24, 2014·BioMed Research International·Nassima DjahmiJean-Philippe Lavigne
Jul 16, 2014·BioMed Research International·Branka BedenićKarmen Godič Torkar
Jul 1, 2015·Open Forum Infectious Diseases·Haley J MorrillKerry L LaPlante
Aug 11, 2015·Journal of Chemotherapy·Abed Zahedi BialvaeiMehdi Yousefi
May 16, 2017·Advanced Biomedical Research·Farzin KhorvashNirvana Tavahen
Oct 17, 2017·Journal of the Chinese Medical Association : JCMA·Zahra HosseinzadehMohammad Motamedifar

❮ Previous
Next ❯

Citations

Sep 6, 2020·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Saya YamasakiMasato Fujisawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

© 2022 Meta ULC. All rights reserved