The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions

Nature Biotechnology
J Nolan, L A Sklar

Abstract

The analysis of macromolecular interactions is an essential element of biomedical research. Flow cytometry is uniquely capable of making sensitive and quantitative measurements of molecular interactions. These measurements can be made in real time with subsecond kinetic resolution using purified biomolecules or living cells. Combined with automated sample handling, these features make flow cytometry a versatile and robust technology for the analysis of molecular interactions.

References

Jan 11, 1980·Science·J C Martin, D E Swartzendruber
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M C NeedelsM A Gallop
Nov 1, 1995·Cytometry·J P NolanL A Sklar
Sep 10, 1996·Biochemistry·J P NolanL A Sklar
Jan 1, 1997·Methods in Enzymology·J J Hill, C A Royer
Oct 23, 1997·Nature Biotechnology·T Misteli, D L Spector

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Citations

Jul 13, 2002·Cytometry·Sabine A Lauer, John P Nolan
Aug 10, 2000·Analytical Biochemistry·X SongB Swanson
Aug 3, 2005·Analytical and Bioanalytical Chemistry·Petra S Dittrich, Andreas Manz
Dec 7, 2013·Molecules and Cells·Morgan Hamon, Jong Wook Hong
Jul 5, 2005·Journal of Immunological Methods·Cecilia A W GeuijenJohn de Kruif
Sep 15, 2000·Journal of Immunological Methods·P S DaughertyG Georgiou
Dec 18, 2001·Trends in Biotechnology·John P Nolan, Larry A Sklar
May 14, 1999·Drug Discovery Today·J P NolanL A Sklar
Sep 5, 2001·Clinical Genetics·N Wedemeyer, T Pötter
Mar 7, 2006·Nature Reviews. Drug Discovery·Petra S Dittrich, Andreas Manz
Nov 22, 2008·Lab on a Chip·Chia-Hsien HsuMehmet Toner
Oct 23, 2010·Lab on a Chip·Sara Lindström, Helene Andersson-Svahn
Feb 3, 2005·Nucleic Acids Research·Jinke WangZuhong Lu
Nov 19, 2013·PloS One·Markus ArnoldiniFrederik Hammes
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dino Di CarloMehmet Toner
Jul 12, 2014·Analytical and Bioanalytical Chemistry·Zhen ZhuAndreas Hierlemann
Dec 3, 2014·Biochimica Et Biophysica Acta·João Miguel FreireMiguel A R B Castanho
Apr 19, 2011·Journal of Bioscience and Bioengineering·Izumi KuboKenji Matoba
May 11, 2010·Biochimica Et Biophysica Acta·Sara Lindström, Helene Andersson-Svahn
Sep 9, 2008·Journal of Immunological Methods·Robert J SchmittlingJohn H Sampson
Nov 9, 2004·Trends in Pharmacological Sciences·Anna WallerLarry A Sklar
Jul 22, 2008·Analytical Biochemistry·Samantha L SchwartzAngela Wandinger-Ness
Feb 25, 2010·Analytical Biochemistry·David J ShermanChristian P Behrenbruch
Dec 4, 2003·Biophysical Journal·Alexandre ChigaevLarry A Sklar
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Larry A SklarEric R Prossnitz
May 30, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Xianjie KangQi Ouyang
Jul 8, 2003·The Journal of Biological Chemistry·Alexandre ChigaevLarry A Sklar
Sep 5, 2008·Current Protocols in Cytometry·B S Edwards, L A Sklar
Feb 1, 2002·Nature Biotechnology·Tomasz Heyduk, Ewa Heyduk
Mar 13, 2015·Lab on a Chip·Xiao WangIan Papautsky
Jun 2, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Loretta Yang, John P Nolan
Jun 2, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Peter C SimonsLarry A Sklar
Aug 6, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yang WuTione Buranda
Nov 11, 2003·Nature Biotechnology·Ahmet ZeytunAndrew R M Bradbury
Apr 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·John P Nolan, Francis Mandy
Mar 22, 2014·Soft Matter·W Neil Everett, Michael A Bevan

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