Jun 24, 2018

The Enchytraeus crypticus stress metabolome - CuO NM case study

Nanotoxicology
Vera L MariaMónica J B Amorim

Abstract

The stress metabolome provides a thorough insight into the signals and hence mechanisms of response of organisms. This is an excellent tool to advance the understanding of interactions, especially for substances like nanomaterials (NMs), for which there is an urgent need for alternative methods for hazard assessment. The metabolome of Enchytraeus crypticus was studied for the first time. The case study, CuO NM (and CuCl2) covered exposure along a time frame [0-7-14 days (d)] and two reproduction effect concentrations (EC10 and EC50). High-performance liquid chromatography-mass spectrometry based method (HPLC-MS) was used, with reversed phase (RP) separation and mass spectrometric detection in positive and negative modes. Metabolite profiling of Cu materials yielded 155 and 382 metabolite features in positive and negative modes, respectively, showing an expression related with time, material, and ECx. The number of differentially expressed metabolites (DEMs) decreased with exposure time (14 d) for CuO NM, whereas for CuCl2 EC50 it increased. Overall, almost all DEMs are down-regulated for CuO NM and up-regulated for CuCl2 (both modes). Early effects were mainly related to amino acids and later to lysophospholipids (down-regulati...Continue Reading

  • References61
  • Citations1

Mentioned in this Paper

Biological Markers
Metabolic Process, Cellular
Copper dioxide (CuO2)
Study
Neuroleptic Malignant Syndrome
Acute-Phase Reaction
High Performance Liquid Chromatography Mass Spectrometry
Energy Transfer
Neutrophil Migration, Function
Amino Acids, I.V. solution additive

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