The end of a polymerizing actin filament contains numerous ATP-subunit segments that are disconnected by ADP-subunits resulting from ATP hydrolysis

Biochemistry
U Pieper, A Wegner

Abstract

ATP hydrolysis by copolymers of ATP-actin and ADP-actin was investigated in order to analyze the effect of interfaces between ATP-subunits and ADP-subunits on hydrolysis of actin-bound ATP. Copolymers of ATP- and ADP-subunits were formed by polymerization of ATP- and ADP-actin monomers onto filaments. By changing the ratio of polymerizing ATP-actin monomers to ADP-actin monomers, the number of interfaces between ATP- and ADP-subunits and of ATP-subunits only surrounded by further ATP-subunits was varied. The rate of actin polymerization and of ATP hydrolysis was measured simultaneously on the same samples. The lag time between incorporation of actin monomers into filaments and subsequent ATP hydrolysis was found to be similar both for polymerized ATP-actin and for copolymers formed by various ratios of ATP- to ADP-actin. The experiments were performed in the presence of 1 mM MgCl2, 0.05 mM CaCl2, and 100 mM KCl or of 1 mM MgCl2, and 0.4 mM EGTA. The type of cations was found to have no major effect on the rate of ATP hydrolysis. A quantitative evaluation of the experimental data suggests that ATP at interfaces between ATP- and ADP-subunits is hydrolyzed not more than 10 times faster than ATP of subunits surrounded by further AT...Continue Reading

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Citations

Mar 3, 2010·Annual Review of Biophysics·Beáta Bugyi, Marie-France Carlier
Feb 28, 2014·The Journal of Physical Chemistry. B·Xin Li, Anatoly B Kolomeisky
Nov 5, 2013·The Journal of Chemical Physics·C Erlenkämper, K Kruse
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dimitrios VavylonisBen O'Shaughnessy
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Feb 19, 2008·Journal of Theoretical Biology·Joseph FassAlex Mogilner
Mar 30, 2012·Biophysical Journal·Ranjith PadinhateeriDavid Lacoste
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Apr 23, 2010·Biophysical Journal·Padinhateeri RanjithDavid Lacoste
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·T D PollardR D Mullins
Nov 2, 2018·Biophysics Reviews·Fikret AydinGregory A Voth
Sep 27, 2018·Biophysical Journal·Harshwardhan H KatkarGregory A Voth
Apr 28, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·F J Brooks, A E Carlsson
Sep 29, 2001·Journal of Molecular Biology·E AndrianantoandroT D Pollard
Nov 13, 2014·The Journal of Physical Chemistry. B·Xin Li, Anatoly B Kolomeisky
Mar 15, 2015·The Journal of Physical Chemistry. B·Xin Li, Anatoly B Kolomeisky
Jul 13, 2013·The Journal of Physical Chemistry. B·Xin Li, Anatoly B Kolomeisky

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