The endocannabinoid system in the human granulosa cell line KGN

Molecular and Cellular Endocrinology
Jana ErnstFaramarz Dehghani

Abstract

Ovarian steroidogenesis is embedded in a sensitive network of regulatory mechanisms crucial for human fertility. The endocannabinoid system (ECS) represents an intrinsic modulating system involved in the regulation of endocrine functions. In the present study we characterized the ECS in the human granulosa cell line KGN and its impact on gonadotropin sensitivity and steroid hormone synthesis under basal and FSH-stimulated conditions. Expression studies were performed and estradiol was measured. CB1, CB2, DAGL, FAAH, GPR55, MAGL, NAPE-PLD and TRPV1 were expressed without FSH-dependent effects. Treatment with selective cannabinoid receptor agonists reduced basal but not FSH-stimulated estradiol and CYP19. Progesterone was not altered by ECS manipulation. CB1 agonist changed the expression of miRNAs associated with granulosa cell function, e.g. miR-23a, miR-24, miR-181a and miR-320a. Present data indicate a modulating role of the intrinsic ovarian ECS in the regulation of estradiol synthesis.

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Citations

May 6, 2016·Cell Adhesion & Migration·Tim HohmannFaramarz Dehghani
Feb 13, 2020·International Journal of Molecular Sciences·Rosaria MeccarielloRiccardo Pierantoni
Apr 22, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Angela Patricia López-CardonaEkaitz Agirregoitia
Jan 23, 2021·International Journal of Molecular Sciences·Antonietta SantoroRosaria Meccariello
Jun 15, 2021·Prostaglandins, Leukotrienes, and Essential Fatty Acids·L CostaB M Fonseca

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