The endocrine disruptor cadmium alters human osteoblast-like Saos-2 cells homeostasis in vitro by alteration of Wnt/β-catenin pathway and activation of caspases

Journal of Endocrinological Investigation
V PapaS Migliaccio

Abstract

The pollutant Cadmium (Cd) is widespread in the environment and causes alterations of human health by acting as an endocrine disruptor. Bone tissue seems to be a crucial target of Cd contamination. Indeed, we have previously demonstrated that this endocrine disruptor induces osteoblast apoptosis and necrosis. Thus, aim of this study was to further evaluate the effect of Cd on osteoblasts homeostasis, investigating potential modification of the Wnt/β-catenin intracellular pathway, the intracellular process involved in programmed cellular death and the cytoskeletal alterations. To this purpose, human osteoblastic Saos-2 cells, a human osteosarcoma osteoblast-like cell line, were cultured and treated with Cd. Osteoblastic cells were treated for 6 h with 10μM Cd, which induced nuclear translocation of β-catenin and increased expression of Wnt/β-catenin target genes. Longer exposure to the same Cd concentration induced osteoblastic cell apoptosis. To better characterize the intracellular events involved in these Cd-induced alterations, we evaluated the effect of Cd exposure on actin filaments and proteins associated to cytoskeletal actin, characterized by the presence of LIM domains. Long (15, 24 h) exposure of osteoblasts to Cd red...Continue Reading

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Citations

Jun 19, 2018·Journal of Toxicology and Environmental Health. Part a·Cristina MonteiroConceição Santos
Dec 7, 2019·Toxicology Mechanisms and Methods·Latifa KnaniSergio Minucci
Mar 7, 2017·Frontiers in Endocrinology·Justin T SmithErin L Hsu
Nov 12, 2016·Journal of Endocrinological Investigation·F CoperchiniL Chiovato
Jan 25, 2019·Journal of Toxicology·Juliana Rodríguez, Patricia Mónica Mandalunis
Jan 28, 2021·Journal of Endocrinological Investigation·V M BimonteS Migliaccio
Apr 17, 2021·International Journal of Hygiene and Environmental Health·Eva OugierJurgen Buekers

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