The endosymbiont (Buchnera sp.) of the aphid Diuraphis noxia contains plasmids consisting of trpEG and tandem repeats of trpEG pseudogenes.

Applied and Environmental Microbiology
C Y LaiN A Moran

Abstract

Most aphids are dependent for their survival on prokaryotic endosymbionts assigned to the genus Buchnera. Among the functions of Buchnera species is the synthesis of tryptophan, which is required by the aphid host. In Buchnera species from the aphid Diuraphis noxia, the genes for anthranilate synthase (trpEG) were found on a plasmid which consisted of seven tandem repeats of a 3.2-kb unit and one 2.6-kb unit which differed in containing a 0.6-kb deletion. One of the 3.2-kb units contained open reading frames corresponding to trpEG; the remaining units contained trpEG pseudogenes (psi). The nucleotide sequence upstream of trpE contained a region that has characteristics of an origin of replication (ori). Relative to trpB (a chromosomal gene), there were about two copies of the trpEG-containing plasmid. Comparisons of the nucleotide sequences of the 3.2-kb units containing trpEG and psi trpEG indicated that most changes occurred in a 700-nucleotide segment that included the region upstream of trpE and the portion of this gene coding for the N terminus. The consequence of these changes was the silencing of trpEG by inactivation of the putative promoter region and premature termination of the TrpE peptide. In contrast, the nucleoti...Continue Reading

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Citations

Jan 1, 1997·Annual Review of Genetics·D Romero, R Palacios
Sep 13, 2005·Annual Review of Microbiology·Paul Baumann
Oct 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nancy A MoranJennifer L Wilcox
Apr 22, 2006·Molecular Ecology·Nancy A Moran, Patrick H Degnan
Feb 27, 2008·Evolution; International Journal of Organic Evolution·Eric M JansonPatrick Abbot
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rosario GilAndrés Moya
Jan 3, 2001·Journal of Bacteriology·J J Wernegreen, N A Moran
Jan 5, 1999·Applied and Environmental Microbiology· Van Ham RCHJA Latorre
Jun 12, 2001·Molecular Phylogenetics and Evolution·J J WernegreenN A Moran
Dec 23, 1999·Current Opinion in Genetics & Development·J O Andersson, S G Andersson
Feb 28, 2001·Microbes and Infection·G GerlachR Gross
Jun 14, 2000·Current Opinion in Microbiology·N A Moran, P Baumann
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Evelyn ZientzRoy Gross
Oct 14, 2004·Genome Research·Emmanuelle Lerat, Howard Ochman

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