The endothelial-to-mesenchymal transition and endothelial cilia in EPC-mediated postischemic kidney protection

American Journal of Physiology. Renal Physiology
Daniel PatschanG A Müller

Abstract

Renal ischemia induces peritubular capillary rarefication and fibrosis, with the latter partly resulting from the endothelial-to-mesenchymal transition (EndoMT). Endothelial cilia transmit blood flow-associated forces into the cell. Early endothelial progenitor cells (eEPCs) have been shown to protect mice from acute kidney injury in the short term. The aim of the present study was to analyze midterm consequences of eEPC treatment in the context of endothelial cilia and the EndoMT. Male C57/Bl6N mice were subjected to unilateral renal ischemia postuninephrectomy. Syngeneic murine eEPCs were systemically injected at the time of reperfusion. Animals were investigated 1, 4, and 6 wk later. Cultured mature endothelial cells were exposed to a variable flow with versus without eEPC supernatant incubation. Systemically injected eEPCs reduced serum creatinine levels at week 1 (35 and 45 min) and week 4 (45 min). Interstitial fibrosis was significantly diminished by cell treatment at all time points as well. The EndoMT was less pronounced at week 4 (35 min) and week 6 (45 min). eEPC supernatant reduced α-smooth muscle actin expression and α-tubulin abundance in flow-treated cultured mature endothelial cells, and percentages of cilium-po...Continue Reading

References

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May 17, 2013·American Journal of Physiology. Renal Physiology·Daniel PatschanGerhard A Müller
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Citations

May 20, 2016·American Journal of Physiology. Renal Physiology·D PatschanG A Müller
Jun 14, 2018·American Journal of Physiology. Renal Physiology·K SchwarzeD Patschan
May 12, 2018·Nature Reviews. Cardiology·Yan LiBin Zhou
Aug 10, 2021·World Journal of Stem Cells·Chee-Yin Wong

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