The endothelin/sarafotoxin-induced increase of the proliferation of undifferentiated and DMSO-differentiated GEM-81 goldfish erythrophoroma cells is mediated by ETB receptors

Pigment Cell Research
Ana Maria Caliman FiladelfiAna Maria de Lauro Castrucci

Abstract

Endothelins (ETs) and sarafotoxins (SRTXs) have been reported to exert ET(B)-mediated effects on vertebrate pigment cells. GEM-81 cell line, a red pigment cell-derived cutaneous tumor of the teleost Carassius auratus, expresses ET(B) receptors and can be differentiated with 1.5% DMSO treatment, thus constituting an useful model to investigate ET and SRTX effects on cultured fish pigment cells. Our aim was to characterize the pharmacology and biological effects mediated by ET receptors in DMSO-differentiated and undifferentiated cells. ET subtype receptors and their respective Ki values in both cell types were determined by competitive binding assays using (125)I ET-1 and BQ-485 (an ET(A) antagonist) or BQ-788 (an ET(B) antagonist). BQ-788, but not BQ-485, significantly reduced (125)I-ET-1 binding in both cell types, with similar low (Ki > nM) affinities. To determine the proliferation effects of ETs/SRTXs, cells were treated for 72 h with the hormones, and counted in a hemocytometer. The proliferation assays were repeated for SRTX S6c in the presence or absence of BQ-788. The results demonstrated that, with the exception of ET-1 (biphasic effect) and ET-3 (no significant effect) in undifferentiated GEM-81 cells, all the tested ...Continue Reading

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Citations

Sep 5, 2009·Chronobiology International·Fernanda Pizão FarhatAna Maria De Lauro Castrucci
May 17, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Guilherme Corrêa RamanziniMaria Aparecida Visconti
Sep 12, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ana Cristina ScarparoAna Maria de Lauro Castrucci
May 13, 2014·General and Comparative Endocrinology·Maria Nathália de Carvalho Magalhães MoraesAna Maria de Lauro Castrucci
Mar 11, 2008·General and Comparative Endocrinology·Adriana LuchsAna Maria de Lauro Castrucci
Aug 31, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G J D LopesA M L Castrucci

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