The energetics of a three-state protein folding system probed by high-pressure relaxation dispersion NMR spectroscopy

Angewandte Chemie
Vitali TugarinovG Marius Clore

Abstract

The energetic and volumetric properties of a three-state protein folding system, comprising a metastable triple mutant of the Fyn SH3 domain, have been investigated using pressure-dependent (15) N-relaxation dispersion NMR from 1 to 2500 bar. Changes in partial molar volumes (ΔV) and isothermal compressibilities (ΔκT ) between all the states along the folding pathway have been determined to reasonable accuracy. The partial volume and isothermal compressibility of the folded state are 100 mL mol(-1) and 40 μL mol(-1)  bar(-1) , respectively, higher than those of the unfolded ensemble. Of particular interest are the findings related to the energetic and volumetric properties of the on-pathway folding intermediate. While the latter is energetically close to the unfolded state, its volumetric properties are similar to those of the folded protein. The compressibility of the intermediate is larger than that of the folded state reflecting the less rigid nature of the former relative to the latter.

References

Jan 1, 1993·Annual Review of Physical Chemistry·J L Silva, G Weber
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S J HagenW A Eaton
May 23, 1998·Biochemistry·K E PrehodaJ L Markley
May 2, 2002·Biochimica Et Biophysica Acta·Nicolas Taulier, Tigran V Chalikian
Jan 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Tigran V Chalikian
Apr 13, 2006·Journal of the American Chemical Society·Dmitry M KorzhnevLewis E Kay
May 11, 2006·Chemical Reviews·Kazuyuki Akasaka
Jun 11, 1982·Science·J Jonas
Nov 16, 2007·The Journal of Physical Chemistry. B·D Flemming HansenLewis E Kay
Mar 31, 2011·Journal of the American Chemical Society·Jean-Baptiste RougetCatherine A Royer
Jul 22, 2011·Journal of the American Chemical Society·Werner KremerHans Robert Kalbitzer
Mar 29, 2012·Journal of the American Chemical Society·Yinan FuA Joshua Wand
Apr 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Julien RocheCatherine A Royer
Apr 21, 2012·Science·Philipp NeudeckerLewis E Kay
Dec 19, 2012·Archives of Biochemistry and Biophysics·Kazuyuki AkasakaYuji O Kamatari
Jan 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Navratna VajpaiStephan Grzesiek
Aug 31, 2013·Journal of the American Chemical Society·Julien RocheCatherine A Royer
Mar 25, 2014·Journal of Magnetic Resonance·Arthur G Palmer
May 7, 2014·The Journal of Physical Chemistry. B·Markus Beck ErlachHans Robert Kalbitzer
Feb 2, 2015·The Journal of Chemical Physics·Fumio Hirata, Kazuyuki Akasaka
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Guilherme A P de Oliveira, Jerson L Silva

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Citations

Oct 25, 2016·Biochemistry·Heinrich KrobathHue Sun Chan
Apr 2, 2017·Journal of Magnetic Resonance·Luan M Nguyen, Julien Roche
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Mengjun XueFrans A A Mulder
Nov 22, 2019·Journal of Biomolecular NMR·Daniel S GarrettG Marius Clore
Oct 5, 2018·Journal of the Royal Society, Interface·Julien Roche, Catherine A Royer
Jan 5, 2017·The Journal of Biological Chemistry·Mayra de A MarquesGuilherme A P de Oliveira
Oct 3, 2018·Journal of Biomolecular NMR·Anusha B GopalanPramodh Vallurupalli
Dec 14, 2019·Current Opinion in Structural Biology·T Reid Alderson, Lewis E Kay
May 8, 2021·Biophysical Journal·Balasubramanian HarishCatherine A Royer

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