PMID: 6987054Jan 1, 1980Paper

The energy-dependent degradation of guanosine 5'-diphosphate 3'-diphosphate in Escherichia coli. Lack of correlation with ATP levels in vivo and role of the transmembrane proton gradient

European Journal of Biochemistry
C TétuP L Boquet

Abstract

Different inhibitors of the energy metabolism have been assayed in Escherichia coli K12 for their ability to increase the level of guanosine 5'-diphosphate 3'-diphosphate (ppGpp) as a consequence of a restriction of its degradation. Inhibitors of the respiration and uncouplers of oxidative phosphorylations had effects similar to carbon-source-induced downshifts while the ATPase inhibitor dicyclohexylcarbodiimide was less efficient. The effects of dicyclohexylcarbodiimide and of the uncoupler carbonylcyanide p-fluoro methoxyphenylhydrazone (FCCP) on ppGpp degradation were compared in a drug-permeable envelope mutant. At concentrations of inhibitors sufficient to deplete the pool of ATP by 50%, only FCCP was able to block ppGpp degradation. Moreover, FCCP also inhibited ppGpp degradation in a ATPase-deficient strain growing on glucose as carbon source while, as expected, it did not change the level of ATP. It is concluded, according to Mitchell's chemiosmotic hypothesis, that, in vivo, the integrity of the transmembrane proton gradient rather than the ATP pool size is a prerequisite for the normal processing of the energy-dependent degradation of ppGpp.

References

May 1, 1976·Cell·A J van OoyenP Jorgensen
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E A Heinemeyer, D Richter
Jan 10, 1977·Biochemical and Biophysical Research Communications·C C PaoJ A Gallant
Feb 15, 1977·Molecular & General Genetics : MGG·C KariA Travers
Jan 1, 1978·Annual Review of Microbiology·D P Nierlich
Feb 1, 1979·Journal of Bacteriology·G Frelat, P L Boquet
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J Sy
Jan 26, 1978·Biochimica Et Biophysica Acta·P Heizmann, S H Howell
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D Richter
Aug 2, 1976·Biochimica Et Biophysica Acta·W J WeyerM Gruber
Sep 1, 1976·Journal of Bacteriology·E E Ishiguro, W D Ramey
Aug 19, 1976·Molecular & General Genetics : MGG·A Travers
Jun 2, 1975·Biochimica Et Biophysica Acta·L Beres, J Lucas-Lenard
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G ReinessM Cashel
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J C StephensB N Ames
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J Sy, F Lipmann
Feb 15, 1972·Biochemistry·R B Harshman, H Yamazaki
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W A Haseltine, R Block
Jul 1, 1972·Journal of Bacteriology·F W Ruscetti, L A Jacobson
Sep 1, 1974·Cell·T Laffler, J A Gallant
Aug 25, 1971·European Journal of Biochemistry·P BoquetP Fromageot
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell

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Citations

Apr 18, 2002·FEMS Microbiology Letters·Rebecca C Greenwood, Daniel R Gentry
Sep 9, 2008·Journal of Bacteriology·Nelli BoesMax Schobert
Jun 4, 2013·International Journal of Food Microbiology·Tineke H JonesLynn M McMullen
Jul 1, 1995·Archives of Microbiology·A BurkovskiR Krämer
Sep 18, 2012·Journal of Photochemistry and Photobiology. B, Biology·Magdalena PezzoniCristina S Costa
Mar 1, 2018·MBio·Liam F FitzsimmonsAndrés Vázquez-Torres
Jan 1, 1983·Zeitschrift für allgemeine Mikrobiologie·K Riedel
Feb 1, 1982·Journal of Bacteriology·J A Akinyanju, R J Smith
Jul 1, 1995·Journal of Bacteriology·D R Gentry, M Cashel
May 15, 1982·Journal of Molecular Biology·J P DavidsonL S Williams

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