PMID: 8951089Oct 1, 1996Paper

The energy transmission in ATP synthase: from the gamma-c rotor to the alpha 3 beta 3 oligomer fixed by OSCP-b stator via the beta DELSEED sequence

Journal of Bioenergetics and Biomembranes
Y Kagawa, T Hamamoto

Abstract

ATP synthase (F0F1) is driven by an electrochemical potential of H+ (delta microH+). F0F1 is composed of an ion-conducting portion (F0) and a catalytic portion (F1). The subunit composition of F1 is a alpha 3 beta 3 gamma delta epsilon. The active alpha 3 beta 3 oligomer, characterized by X-ray crystallography, has been obtained only from thermophilic F1 (TF1). We proposed in 1984 that ATP is released from the catalytic site (C site) by a conformational change induced by the beta DELSEED sequence via gamma delta epsilon-F0. In fact, cross-linking of beta DELSEED to gamma stopped the ATP-driven rotation of gamma in the center of alpha 3 beta 3. The torque of the rotation is estimated to be 420 pN x A from the delta microH+ and H(+)-current through F0F1. The angular velocity (omega) of gamma is the rate-limiting step, because delta microH+ increased the Vmax of H+ current through F0, but not the Km(ATP). The rotational unit of F0 (= ab2c10) is pi/5, while that in alpha 3 beta 3 is 2 pi/3. This difference is overcome by an analog-digital conversion via elasticity around beta DELSEED with a threshold to release ATP. The alpha beta distance at the C site is about 9.6 A (2,8-diN3-ATP), and tight Mg-ATP binding in alpha 3 beta 3 gamma...Continue Reading

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Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·Shinji MinoharaNobuhito Sone
Nov 18, 2008·Nature Structural & Molecular Biology·Tomoko MasaikeTakayuki Nishizaka
Sep 27, 2012·The Journal of Biological Chemistry·Ei-Ichiro SunamuraToru Hisabori
Aug 7, 2010·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yasuo Kagawa
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·E J BoekemaW N Konings
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·T D BunneyA H de Boer
Dec 13, 2003·Biochimica Et Biophysica Acta·Paul D GavinMark Prescott
May 9, 2000·The Journal of Biological Chemistry·K Y HaraM Yoshida
Dec 6, 1997·Biochemical and Biophysical Research Communications·Y Kagawa, T Hamamoto
Aug 27, 1999·Advances in Biophysics·Y Kagawa

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