The enhanced anti-angiogenic and antitumor effects of combining flk1-based DNA vaccine and IP-10

Vaccine
Xiao-ling LuSheng-min Zhang

Abstract

The purpose of the present study was to evaluate the anti-vasculature effects and the anti-tumor effects of attenuated Salmonella typhimurium vaccine strain encoding murine vascular endothelial growth factor (VEGF) receptor-2 (flk1) in combination with plasmid DNA vector encoding the murine interferon-induced protein of 10kDa (IP-10 or CXCL10) gene. Mouse models of malignant melanoma (B16-F10) were treated with combining orally given attenuated S. typhimurium vaccine strain encoding flk1 with direct intratumoral injection of a non-viral plasmid DNA vector encoding the murine IP-10 gene. The volumes of tumors and survival of mice bearing B16-F10 tumors were observed. Cytolytic T lymphocyte (CTL) response was measured by cytotoxic assay, vessel density and tumor-cell proliferation were observed by immunostaining, and tumor apoptosis was determined by TUNEL staining. The results revealed the combination therapy groups showed more significantly inhibited tumor growth, apoptosis of tumor cells, and reduced neovascularization, cell proliferation, and developed a strong CTL response in these mice. In summary, the therapy of attenuated S. typhimurium vaccine strain encoding flk1 combined with the IP-10 gene has significant synergistic ...Continue Reading

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Citations

Sep 6, 2012·Immunotherapy·Elham Mohit, Sima Rafati
Jan 8, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Matilda RentoftKarin Nylander
Feb 9, 2012·Expert Review of Vaccines·Fadi Saade, Nikolai Petrovsky
Feb 19, 2009·Pigment Cell & Melanoma Research·Ann RichmondYingjun Su
Dec 17, 2014·Cytokine & Growth Factor Reviews·Katrien Van RaemdonckSofie Struyf
Feb 3, 2015·Biochimica Et Biophysica Acta·Madelon Q WentinkArjan W Griffioen
Oct 1, 2016·Chonnam Medical Journal·Jin Hai Zheng, Jung-Joon Min
Apr 19, 2017·Journal of Surgical Oncology·Nancy D Ebelt, Edwin R Manuel
Oct 16, 2013·Expert Review of Vaccines·Bertrand ToussaintAudrey Le Gouëllec
Aug 1, 2012·Oncology Letters·Mingli LiuJonathan K Stiles
Aug 22, 2018·Annals of Clinical and Translational Neurology·Margaret J LandreneauUNKNOWN Serum Markers After Spontaneous Cerebral Hemorrhage (SMASCH) Investigators
Oct 23, 2020·Pigment Cell & Melanoma Research·Tayebeh RezaeiAhad Mokhtarzadeh
Jun 14, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Huiqin ZhuoYongxiang Zhao

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