The enhanced antitumour response of pimozide combined with the IDO inhibitor L‑MT in melanoma

International Journal of Oncology
Huijie JiaZhongwei Tian

Abstract

Melanoma is one of the most fatal and therapy-resistant types of cancer; therefore, identifying novel therapeutic candidates to improve patient survival is an ongoing effort. Previous studies have revealed that pimozide is not sufficient to treat melanoma; therefore, enhancing the treatment is necessary. Indoleamine 2, 3‑dioxygenase (IDO) is an immunosuppressive, intracellular rate-limiting enzyme, which contributes to immune tolerance in various tumours, including melanoma, and inhibition of IDO may be considered a novel therapeutic strategy when combined with pimozide. The present study aimed to assess the antitumour activities of pimozide in vitro, and to investigate the effects of pimozide combined with L‑methyl-tryptophan (L‑MT) in vivo. For in vitro analyses, the B16 melanoma cell line was used. Cell cytotoxicity assay, cell viability assay, wound‑healing assay and western blotting were conducted to analyse the effects of pimozide on B16 cells. Furthermore, B16 cell-bearing mice were established as the animal model. Haematoxylin and eosin staining, immunohistochemistry, terminal deoxynucleotidyl transferase dUTP nick end-labelling staining, western blotting and flow cytometry were performed to determine the effects of mon...Continue Reading

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Citations

Feb 13, 2020·Cancers·Carla BarcelóAnna Macià
Jan 26, 2021·Frontiers in Oncology·Hernán CortésGerardo Leyva-Gómez
May 19, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·R ZouS Cui
Jul 3, 2021·Pharmaceuticals·Adrian MorDariusz Pawlak

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Methods Mentioned

BETA
pharmacotherapies
light microscopy
protein assay
xenograft

Software Mentioned

Quantity One
SPSS

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