The enhancement of Cx45 expression and function in renal interlobar artery of spontaneously hypertensive rats at different age

Kidney & Blood Pressure Research
Li LiJun-Qiang Si

Abstract

This study was designed to investigate the expression and function of gap junction protein connexin 45 (Cx45) in renal interlobar artery (RIA) of spontaneously hypertensive rats (SHR), and the association between hypertension and enhanced vasoconstrictive response in SHR. Western blot analysis and pressure myography were used to examine the differences in expression and function of Cx45 in vascular smooth muscle cells (VSMCs) of RIA between SHR and normotensive Wistar-Kyoto (WKY) rats. Our results demonstrated that 1) whole-cell patch clamp measurements showed that the membrane capacitance and conductance of in-situ RIA VSMCs of SHR were significantly greater than those of WKY rats (p<0.05, n=6), suggesting that the coupling of gap junction between VSMCs of RIA was enhanced in SHR; 2) the KCl or phenylephrine (PE)-stimulated RIA constriction was more pronounced in SHR than that in WKY rats (p<0.05, n=10). After applying a gap junction inhibitor 18β-glycyrrhetintic acid (18β-GA), the inhibitory effect of 18β-GA on KCl or PE-induced vasoconstriction was greater in SHR (p<0.05, n=10); and 3) the expression of Cx45 in RIA of SHR was greater than that in WKY rats (p<0.05, n=3) at 4, 12 and 48 wks of age. The hypertension-induced ele...Continue Reading

Citations

Mar 21, 2012·PloS One·Vishal M ShindeMarina S Gorbatyuk
Jul 9, 2016·Critical Reviews in Biochemistry and Molecular Biology·Joost WillebrordsMathieu Vinken
Jun 3, 2018·Acta Physiologica·K PogodaU Pohl
Jul 6, 2017·Molecular Medicine Reports·Yan-Fei QianJun-Qiang Si
Jan 26, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Hao WangJing-Song Xu
Nov 17, 2020·American Journal of Physiology. Renal Physiology·Yan XuSebastian Bachmann
Feb 3, 2021·International Journal of Molecular Sciences·Mirela LozićKatarina Vukojević
Feb 15, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Shlok JindalGopinath Packirisamy
Jun 1, 2021·American Journal of Physiology. Renal Physiology·Jenny VoggelKai-Dietrich Nüsken

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