PMID: 9557708Apr 29, 1998Paper

The envelope protein encoded by the A33R gene is required for formation of actin-containing microvilli and efficient cell-to-cell spread of vaccinia virus

Journal of Virology
R L RoperB Moss

Abstract

The vaccinia virus (VV) A33R gene encodes a highly conserved 23- to 28-kDa glycoprotein that is specifically incorporated into the viral outer envelope. The protein is expressed early and late after infection, consistent with putative early and late promoter sequences. To determine the role of the protein, two inducible A33R mutants were constructed, one with the late promoter and one with the early and late A33R promoter elements. Decreased A33R expression was associated with small plaques that formed comets in liquid medium. Using both an antibiotic resistance gene and a color marker, an A33R deletion mutant, vA33delta, was isolated, indicating that the A33R gene is not essential for VV replication. The plaques formed by vA33delta, however, were tiny, indicating that the A33R protein is necessary for efficient cell-to-cell spread. Rescue of the large-plaque phenotype was achieved by inserting a new copy of the A33R gene into the thymidine kinase locus, confirming the specific genetic basis of the phenotype. Although there was a reduction in intracellular virus formed in cells infected with vA33delta, the amount of infectious virus in the medium was increased. The virus particles in the medium had the buoyant density of extrac...Continue Reading

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Citations

Feb 17, 2000·The Journal of General Virology·D BoulangerM A Skinner
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·M B Goldberg
Dec 12, 2001·Journal of Virology·David C Johnson, Mary T Huber
Feb 1, 2005·Journal of Virology·Susana GuerraMariano Esteban
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mansun LawGeoffrey L Smith
Aug 17, 2006·Journal of Virology·Beatriz Perdiguero, Rafael Blasco
Nov 18, 2011·Journal of Virology·Katja SlivaBarbara S Schnierle
May 25, 2012·The Journal of General Virology·Virginie DoceulGeoffrey L Smith
Sep 19, 2002·The Journal of General Virology·Oliver KraussGeoffrey L Smith
Dec 6, 2002·The Journal of General Virology·Geoffrey L SmithMansun Law
Apr 28, 2006·Journal of Virology·C L AfonsoD L Rock
Jun 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Herman W FavoreelHans J Nauwynck
Sep 24, 2010·Future Microbiology·Clement A Meseda, Jerry P Weir
Jul 27, 2001·The Journal of Cell Biology·M HollinsheadG L Smith
Dec 21, 2012·The Journal of General Virology·Adrien BreimanGeoffrey L Smith
Jul 14, 2010·Médecine sciences : M/S·Virginie DoceulGeoffrey L Smith
Apr 20, 2012·Journal of Virology·Chad R Irwin, David H Evans

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