PMID: 6414330Jul 1, 1983Paper

The enzymatic determination of bicarbonate and CO2 in reagents and buffer solutions

Analytical Biochemistry
N P HallA J Keys

Abstract

A method for the determination of bicarbonate in buffer solutions between pH 7.5 and 8.75 and in stock solutions of NaHCO3 is described. The HCO-3 is reacted with phosphoenolpyruvate (PEP) in the presence of PEP carboxylase (EC 4.1.1.31) and the oxaloacetate formed reduced to malate by NADH in the reaction catalyzed by malate dehydrogenase (EC 1.1.1.37). The extent of oxidation of NADH is measured spectrophotometrically. Experiments using standard solutions show that 1 mol of NADH is oxidized per mol of HCO-3 added. The method was used to establish the precautions needed to prepare buffer solutions containing less than 1% of the bicarbonate which would be present in the same buffers in equilibrium with air.

References

Jul 1, 1977·Archives of Biochemistry and Biophysics·S K Mukerji
Jul 1, 1974·Archives of Biochemistry and Biophysics·H M MiziorkoA S Mildvan

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Citations

Mar 1, 1989·The Annals of Otology, Rhinology, and Laryngology·J H IsaacsT H Maren
Jul 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M S Anwer, K Nolan
May 18, 2006·American Journal of Physiology. Renal Physiology·Yuehan ZhouWalter F Boron

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