The Epigenetic Factor KDM2B Regulates EMT and Small GTPases in Colon Tumor Cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Nefeli ZacharopoulouChristos Stournaras

Abstract

The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed its role in regulating EMT and small GTPases expression in colon tumor cells. We used RT-PCR for the transcriptional analysis of various genes, Western blotting for the assessment of protein expression and immunofluorescence microscopy for visualization of fluorescently labeled proteins. We report here that KDM2B regulates EZH2 and BMI1 in HCT116 colon tumor cells. Knockdown of this epigenetic factor induced potent up-regulation of the protein levels of the epithelial markers E-cadherin and ZO-1, while the mesenchymal marker N-cadherin was downregulated. On the other hand, KDM2B overexpression downregulated the levels of both epithelial markers and upregulated the mesenchymal marker, suggesting control of EMT by KDM2B. In addition, RhoA, RhoB and RhoC protein levels diminished upon KDM2B-knockdown, while all three small GTPases became upregulated in KDM2B-overexpressing HCT116 cell clones. Interestingly, Rac1 GTPase level increased upon KDM2B-knockdow...Continue Reading

Citations

Mar 17, 2020·Cancer Biology & Therapy·Nefeli ZacharopoulouChristos Stournaras
Jun 21, 2019·Journal of Cellular and Molecular Medicine·Xin Shi, Mingfu Fan
Nov 28, 2019·Scientific Reports·Ellen WikeniusAnders Berglund
Sep 19, 2019·Journal of Cellular Physiology·Yuxi Qi, Yanning Zhao
Apr 2, 2021·Frontiers in Oncology·Jaceline Gisliane Pires SanchesLianhong Li

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