Abstract
The post translational modification of lysine acetylation is a key mechanism that regulates chromatin structure. Epigenetic readers, such as the BET domains, are responsible for reading histone lysine acetylation which is a hallmark of open chromatin structure, further providing a scaffold that can be accessed by RNA polymerases as well as transcription factors. Recently, several reports have assessed and highlighted the roles of epigenetic readers in various cellular contexts. However, little is known about their role in the regulation of inflammatory genes, which is critical in exquisitely tuning inflammatory responses to a variety of immune stimuli. In this study, we investigated the role of epigenetic readers BRD2 and BRD4 in the lipopolysaccharide (LPS)-induced immune responses in mouse primary astrocytes. Inflammatory stimulation by LPS showed that the levels of Brd2 mRNA and protein were increased, while Brd4 mRNA levels did not change. Knocking down of Brd2 mRNA using specific small interfering RNA (siRNA) in cultured mouse primary astrocytes inhibited LPS-induced mRNA expression and secretion of plasminogen activator inhibitor-1 (PAI-1). However, no other pro-inflammatory cytokines, such as Il-6, Il-1β and Tnf-α, were ...Continue Reading
References
Feb 1, 1996·Genes & Development·G V Denis, M R Green
May 29, 1998·Immunogenetics·K L Thorpe, S Beck
Jun 1, 2001·Brain Research·C Y ShinK H Ko
Jun 20, 2001·The Journal of Experimental Medicine·S SaccaniG Natoli
Dec 20, 2002·Nature·Carl Nathan
Oct 21, 2004·Glia·Iordanis Gravanis, Stella E Tsirka
Nov 19, 2004·The Biochemical Journal·Anupama SinhaGerald V Denis
Apr 21, 2005·Thrombosis and Haemostasis·Jerry P Melchor, Sidney Strickland
May 23, 2007·Molecular and Cellular Biology·K E SullivanM Bhatia
May 25, 2007·Nature·Andrew P Feinberg
Aug 22, 2007·Neurology·Eduardo E Benarroch
Dec 24, 2008·Molecular and Cellular Biology·Bo HuangLin-Feng Chen
Jun 6, 2009·Journal of Dental Research·S P Barros, S Offenbacher
Nov 4, 2009·The Biochemical Journal·Fangnian WangGerald V Denis
Nov 10, 2009·Nature Genetics·Hiba MahdiKarin Lundberg
Sep 28, 2010·Nature·Panagis FilippakopoulosJames E Bradner
Nov 12, 2010·Nature·Edwige NicodemeAlexander Tarakhovsky
Jan 5, 2011·Neurochemistry International·Ji Woon KimChan Young Shin
Jan 20, 2011·Genes & Development·Gioacchino NatoliIros Barozzi
May 25, 2011·Journal of Cellular Biochemistry·Enyuan ShangDebra J Wolgemuth
Jul 18, 2012·Thrombosis Research·Britt Van De CraenAnn Gils
Jul 24, 2012·Cell·Dev M BhattStephen T Smale
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·William W LockwoodHarold Varmus
Feb 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna C BelkinaGerald V Denis
Apr 16, 2013·Cell·Jakob LovénRichard A Young
Sep 21, 2013·Molecular Biology of the Cell·Jarmila HnilicováDavid Staněk
Nov 20, 2013·Molecular and Cellular Biology·Sebastian WienerroitherThomas Decker
Apr 23, 2014·Nature Reviews. Drug Discovery·Panagis Filippakopoulos, Stefan Knapp
Apr 25, 2014·Nature·Irfan A AsanganiArul M Chinnaiyan
Aug 12, 2014·Molecular Neurobiology·Hyun Myung KoChan Young Shin
Citations
Aug 6, 2016·Cellular and Molecular Life Sciences : CMLS·Dequina A NicholasGerald V Denis
Jan 13, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Suzanne AcklooSusanne Müller
Apr 27, 2016·Current Opinion in Clinical Nutrition and Metabolic Care·Dashzeveg Bayarsaihan
Jan 20, 2021·Signal Transduction and Targeted Therapy·Nian WangRui Kang
Mar 30, 2021·Frontiers in Pharmacology·Nian WangDaolin Tang