The epigenetic signature of subcutaneous fat cells is linked to altered expression of genes implicated in lipid metabolism in obese women

Clinical Epigenetics
P ArnerIngrid Dahlman

Abstract

Obesity is associated with changes in fat cell gene expression and metabolism. What drives these changes is not well understood. We aimed to explore fat cell epigenetics, i.e., DNA methylation, as one mediator of gene regulation, in obese women. The global DNA methylome for abdominal subcutaneous fat cells was compared between 15 obese case (BMI 41.4 ± 4.4 kg/m(2), mean ± SD) and 14 never-obese control women (BMI 25.2 ± 2.5 kg/m(2)). Global array-based transcriptome analysis was analyzed for subcutaneous white adipose tissue (WAT) from 11 obese and 9 never-obese women. Limma was used for statistical analysis. We identified 5529 differentially methylated DNA sites (DMS) for 2223 differentially expressed genes between obese cases and never-obese controls (false discovery rate <5 %). The 5529 DMS displayed a median difference in beta value of 0.09 (range 0.01 to 0.40) between groups. DMS were under-represented in CpG islands and in promoter regions, and over-represented in open sea-regions and gene bodies. The 2223 differentially expressed genes with DMS were over-represented in key fat cell pathways: 31 of 130 (25 %) genes linked to "adipogenesis" (adjusted P = 1.66 × 10(-11)), 31 of 163 (19 %) genes linked to "insulin signaling"...Continue Reading

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Citations

Mar 2, 2016·The Journal of Physiology·Laura M PérezBeatriz G Gálvez
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Datasets Mentioned

BETA
GSE67024

Methods Mentioned

BETA
methylation profiling
ELISA
Assay
Methylation
PCR

Clinical Trials Mentioned

NCT01785134

Software Mentioned

Bioconductor Lumi
BMIQ
GenomeStudio
Webgestalt
Bioconductor package
Limma
GenomeStudio Methylation module
Affymetrix Expression Console

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