The epithelial brush border Na+/H+ exchanger NHE3 associates with the actin cytoskeleton by binding to ezrin directly and via PDZ domain-containing Na+/H+ exchanger regulatory factor (NHERF) proteins

Clinical and Experimental Pharmacology & Physiology
Boyoung Cha, M Donowitz

Abstract

1. The Na(+)/H(+) exchanger NHE3 associates with the actin cytoskeleton by binding ezrin both directly and indirectly. Both types of interaction are necessary for acute regulation of NHE3. Most acute regulation of NHE3 occurs by changes in trafficking via effects on exocytosis and/or endocytosis. However, NHE3 activity can also be regulated without changing the surface expression of NHE3 (change in turnover number). 2. A positive amino acid cluster in the a-helical juxtamembrane region in the COOH-terminus of NHE3 (amino acids K516, R520 and R527) is necessary for binding to the protein 4.1, ezrin, radixin, moesin (FERM) domain III of ezrin. Direct binding of NHE3 to ezrin is necessary for many aspects of basal trafficking, including basal exocytosis, delivery from the synthetic pathway and movement of NHE3 in the brush border (BB), which probably contributes to endocytosis over a prolonged period of time. 3. In addition, NHE3 binds indirectly to ezrin. The PDZ domain-containing proteins Na(+)/H(+) exchanger regulatory factor (NHERF) 1 and NHERF2, as intermediates in linking NHE3 to ezrin, are necessary for many aspects of NHE3 regulation. The binding of NHERF-ezrin/radixin/moesin to NHE3 occurs in the cytosolic domain of NHE3 ...Continue Reading

References

Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·C H YunM Donowitz
Aug 7, 1998·Molecular Biology of the Cell·T Tominaga, D L Barber
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·A GautreauM Arpin
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A BretscherD Reczek
Jul 18, 2001·The Journal of Clinical Investigation·T TakedaM G Farquhar
Jul 20, 2001·Journal of the American Society of Nephrology : JASN·Robert A OrlandoMarilyn G Farquhar
Oct 16, 2001·American Journal of Physiology. Cell Physiology·J R Turner, E D Black
Aug 3, 2002·Nature Reviews. Molecular Cell Biology·Anthony BretscherRichard G Fehon
Dec 18, 2002·The Journal of Cell Biology·Sheryl P Denker, Diane L Barber
Sep 5, 2003·American Journal of Physiology. Cell Physiology·Whaseon Lee-KwonMark Donowitz
Jan 6, 2004·Nature Medicine·Chand KhannaLee J Helman
Apr 14, 2004·The Journal of General Physiology·Hisayoshi HayashiSergio Grinstein
Apr 21, 2004·The Journal of Biological Chemistry·Karen L WuJeffrey R Schelling
May 4, 2004·Trends in Molecular Medicine·Kent W Hunter
Jun 5, 2004·Developmental Cell·Ichiko SaotomeAndrea I McClatchey
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Huiren ZhaoJerrold R Turner
Sep 2, 2004·Journal of the American Society of Nephrology : JASN·Sandra SchmiederMarilyn G Farquhar
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Martin BaumgartnerDiane L Barber
Nov 9, 2004·The Journal of Biological Chemistry·Harn ShiueJerrold R Turner

❮ Previous
Next ❯

Citations

Aug 6, 2009·Current Opinion in Nephrology and Hypertension·Xiaoyan WangPedro A Jose
Mar 25, 2011·American Journal of Physiology. Cell Physiology·Rakhilya MurtazinaMark Donowitz
May 19, 2009·The Journal of Experimental Biology·Mark DonowitzXuhang Li
Jun 27, 2009·Journal of the American Society of Nephrology : JASN·Francesca Di SoleOrson W Moe
Jan 5, 2011·Brain Research·Amy L UhernikJeffrey P Smith
Aug 12, 2015·The American Journal of Pathology·Kavisha AroraAnjaparavanda P Naren
Mar 16, 2016·Biochimica Et Biophysica Acta·Paleerath PeerapenVisith Thongboonkerd
Aug 11, 2015·The Journal of Clinical Investigation·Peijian HeC Chris Yun
Feb 9, 2012·International Journal of Cell Biology·Silvia Chifflet, Julio A Hernández
Mar 14, 2014·Science Translational Medicine·Andrew G SpencerDominique Charmot
Sep 24, 2014·Experimental Biology and Medicine·Pei WangZequan Ji
May 13, 2017·Molecular Biology of the Cell·Rafiquel SarkerMark Donowitz
Jun 7, 2014·Circulation Research·Brandon A KempRobert M Carey
Feb 18, 2011·Gut Microbes·Kim Hodges, Ravinder Gill
Apr 23, 2021·The American Journal of Pathology·Yoshiyasu FukusumiHiroshi Kawachi
May 19, 2009·The Journal of Experimental Biology·R Todd Alexander, Sergio Grinstein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.