PMID: 9177218Jun 10, 1997Paper

The epitopes for natural polyreactive antibodies are rich in proline

Proceedings of the National Academy of Sciences of the United States of America
B TchernychevM Wilchek

Abstract

"Natural" polyreactive antibodies, which bind in a nonspecific manner to a range of biological molecules both of self- and nonself- origin, are normal constituents of serum and are a significant part of the immune repertoire in many species, including humans. Autoantibodies to sTNF-R (the 55-kDa extracellular domain of the human receptor to tumor necrosis factor alpha) were affinity purified from normal human sera using immobilized sTNF-R. The isolated anti-sTNF-R IgG bound both native and denatured forms of the receptor with low affinity. These antibodies also bound to different proteins and therefore are considered to be polyreactive. We used the anti-sTNF-R antibodies and purified polyreactive antibodies to mannose-specific lectin from garlic (Allium sativum) for screening a peptide library displayed on filamentous M13 phage. After the biopanning procedure, we failed to find epitopes with a consensus sequence; however, we found that proline is the most frequent amino acid in the selected phagotopes. Proline is commonly present at solvent-exposed sites in proteins, such as loops, turns, N-terminal first turn of helix, and random coils. Thus, structures containing proline can serve as conformation-dependent common "public" epi...Continue Reading

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Citations

Aug 11, 1998·Immunology Letters·A AmiciF M Venanzi
Jun 25, 1999·Journal of Virological Methods·K C GoughG C Whitelam
Apr 18, 2003·Biology of Reproduction·Karen D CarpenterThomas E Spencer
Feb 13, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·K BurgessH U Saragovi
Jul 28, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bergljot MagnadottirSigurdur Helgason
Oct 14, 2008·Journal of Autoimmunity·Brian D PooleJudith A James
Nov 6, 2007·Fish & Shellfish Immunology·Shona K Whyte
Jun 14, 2005·Fish & Shellfish Immunology·Bergljót Magnadóttir
Feb 9, 2012·Journal of Biomedicine & Biotechnology·Sulabha PathakShobhona Sharma
Jul 21, 2016·Veterinary Immunology and Immunopathology·Stefano CecchiniAnna Rocchina Caputo
Oct 5, 2016·Journal of Proteome Research·Elisa PinJochen M Schwenk
Apr 30, 2002·International Immunology·Anastas PashovSrini V Kaveri
Dec 19, 2019·Frontiers in Immunology·Anastas PashovEivind Hovig

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