PMID: 11911588Mar 26, 2002Paper

The epizootic haematopoietic necrosis virus (Iridoviridae) induces apoptosis in vitro

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
S Essbauer, W Ahne

Abstract

The epizootic haematopoietic necrosis virus (EHNV) is an iridovirus causing severe disease in different fish species. We investigated the induction of apoptosis during EHNV infection of the epithelioma carp papulosum (EPC) cell line. Apoptosis reveals several characteristic morphological changes, such as chromatin condensation, nuclear fragmentation, cytoplasm membrane disorientation, or mitochondrial changes. During EHNV infection of EPC cells the occurrence of apoptosis was analysed using a fluorescein-isothiocyanate (FITC) conjugate of annexin-V to detect phosphatidylserines that have changed cytoplasm membrane localization. Annexin-V labelling was obvious 12 h after infection. At 54 h after EHNV infection 39% of the investigated EPC cells exhibited fluorescence. Furthermore, EHNV-infected cells were stained with 4'-6'-diamidino-2-phenylindole (DAPI) to detect pycnotic nuclei. Appearance of DAPI-positive nuclei was found beginning at 18 h after infection. At 54 h after EHNV infection approximately 56% of the EPC cells showed fragmented nuclei. Assays to inhibit a protein kinase-dependent (e.g. double-stranded RNA-dependent protein kinase) apoptosis pathway with 2-aminopurine revealed a reduction of EHNV titres, e.g. titres w...Continue Reading

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Citations

Jun 7, 2007·Apoptosis : an International Journal on Programmed Cell Death·You-Hua HuangQi-Ya Zhang
Oct 24, 2007·Apoptosis : an International Journal on Programmed Cell Death·Pei-Wen LinChi-Yao Chang
Mar 29, 2011·Apoptosis : an International Journal on Programmed Cell Death·Youhua HuangQiwei Qin
Oct 14, 2011·Journal of Virology·Nilesh S ChitnisShan L Bilimoria
Apr 15, 2004·Australian Veterinary Journal·M J LancasterC J Schroen
Mar 19, 2003·Virology·V G ChincharG D Chinchar
Mar 10, 2007·Archives of Virology·E R PaulS L Bilimoria

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis