The Epstein-Barr virus BMRF1 gene is essential for lytic virus replication.

Journal of Virology
Bernhard Neuhierl, Henri-Jacques Delecluse

Abstract

The Epstein-Barr virus (EBV) BMRF1 protein is a DNA polymerase processivity factor. We have deleted the BMRF1 open reading frame from the EBV genome and assessed the DeltaBMRF1 EBV phenotype. DeltaBMRF1 viruses were replication deficient, but the wild-type phenotype could be restored by BMRF1 trans-complementation. The replication-deficient phenotype included impaired lytic DNA replication and late protein expression. DeltaBMRF1 and wild-type viruses were undistinguishable in terms of their ability to transform primary B cells. Our results provide genetic evidence that BMRF1 is essential for lytic replication of the EBV genome.

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Citations

Sep 18, 2009·Journal of Virology·Fu-Zhang WangJoseph S Pagano
Jan 1, 2010·Herpesviridae·Regina FeederleHenri-Jacques Delecluse
Jan 1, 2010·Future Virology·Andrew J Rennekamp, Paul M Lieberman
Aug 27, 2015·Journal of Medical Virology·Sameer LapsiaSumita Bhaduri-McIntosh
Jun 10, 2015·Virology·Takahiro WatanabeTakayuki Murata
Aug 15, 2009·PLoS Pathogens·Roland HutzingerAlexander Hüttenhofer
Mar 8, 2011·PLoS Pathogens·Regina FeederleHenri-Jacques Delecluse
Jul 15, 2017·Hematological Oncology·Melina CohenPaola Andrea Chabay
Oct 26, 2018·Journal of Virology·Vladimir MajerciakZhi-Ming Zheng
Aug 17, 2020·Journal of Virology·Xiaofan LiSumita Bhaduri-McIntosh
Aug 23, 2020·Microbiology and Immunology·Anna Lystia PoetrantoYasuko Mori
Mar 30, 2021·Virus Research·Beata Cohan, Lori Frappier
Jul 2, 2021·Infectious Agents and Cancer·Francisco AguayoRancés Blanco

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