The Epstein-Barr Virus Lytic Protein BZLF1 as a Candidate Target Antigen for Vaccine Development

Cancer Immunology Research
Alex S HartlageRobert A Baiocchi

Abstract

The Epstein-Barr virus (EBV) is an oncogenic, γ-herpesvirus associated with a broad spectrum of disease. Although most immune-competent individuals can effectivley develop efficient adaptive immune responses to EBV, immunocompromised individuals are at serious risk for developing life-threatening diseases, such as Hodgkin lymphoma and posttransplant lymphoproliferative disorder (PTLD). Given the significant morbidity associated with EBV infection in high-risk populations, there is a need to develop vaccine strategies that restore or enhance EBV-specific immune responses. Here, we identify the EBV immediate-early protein BZLF1 as a potential target antigen for vaccine development. Primary tumors from patients with PTLD and a chimeric human-murine model of EBV-driven lymphoproliferative disorder (EBV-LPD) express BZLF1 protein. Pulsing human dendritic cells (DC) with recombinant BZLF1 followed by incubation with autologous mononuclear cells led to expansion of BZLF1-specific CD8(+) T cells in vitro and primed BZLF1-specific T-cell responses in vivo. In addition, vaccination of hu-PBL-SCID mice with BZLF1-transduced DCs induced specific cellular immunity and significantly prolonged survival from fatal EBV-LPD. These findings ident...Continue Reading

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Citations

Oct 10, 2015·Current Hematologic Malignancy Reports·A A GruP Porcu
Feb 6, 2020·Vaccines·Dejan JakimovskiRobert Zivadinov
Jun 11, 2020·Cancers·Diego GerminiYegor Vassetzky
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Jul 10, 2021·Frontiers in Microbiology·Vincent Jean-PierreRaphaële Germi
Jun 26, 2021·Frontiers in Immunology·Cong SunMu-Sheng Zeng

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