The equine herpesvirus 1 IR6 protein influences virus growth at elevated temperature and is a major determinant of virulence

Virology
Nikolaus OsterriederD J O'Callaghan

Abstract

The diploid IR6 gene (ORF 67) of equine herpesvirus type 1 (EHV-1) is absent in the modified live EHV-1 vaccine strain RacH and is present in a mutated form in the avirulent EHV-1 strains RacM24 and RacM36, such that the IR6 protein fails to form the typical rod-like structures observed for wild-type EHV-1 RacL11. To assess the role of the IR6 protein in EHV-1 replication and virulence, two recombinant RacH viruses, HIR6-1 and HIR6-2, that harbor a single copy of the wild-type IR6 gene were engineered and characterized. It was shown that: (i) HIR6-1 or HIR6-2 virus encoded for an IR6 protein that was capable of forming the rod-like structures typical of cells infected with the wild-type virulent virus strain RacL11. (ii) Whereas the avirulent EHV-1 strains RacH and RacM36 are temperature-sensitive (in that virus replication at 40 degrees versus that at 37 degrees was reduced by as much as 7,500-fold), the HIR6-1 and HIR6-2 viruses, like RacL11 virus, were capable of significant replication at the elevated temperature. (iii) Electron microscopic analyses revealed that cells infected with the HIR6-1 or HIR6-2 virus, like those infected with virulent RacL11 virus, produced and released comparable numbers of virus particles at both...Continue Reading

Citations

Sep 10, 2010·Journal of Virology·Helga Hofmann-SieberRalf Wagner
Mar 27, 2001·Journal of Virology·H GranzowT C Mettenleiter
Sep 13, 2003·Journal of Virology·G DelhonD L Rock
Feb 15, 2011·Veterinary Research·Gisela Soboll HusseyDavid P Lunn
Jan 1, 2002·The Veterinary Quarterly·C van Maanen
Feb 3, 2006·Expert Review of Vaccines·Cristina T RosasNikolaus Osterrieder
Jun 7, 2007·The Journal of Veterinary Medical Science·Yasser M GhanemHideto Fukushi
Nov 22, 2017·BMC Genomics·Aaron W KolbCurtis R Brandt
Jun 10, 1998·Virus Research·E J Haanes, C C Tomlinson

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