The ERA-Related GTPase AtERG2 Associated with Mitochondria 18S RNA Is Essential for Early Embryo Development in Arabidopsis

Frontiers in Plant Science
Pengyu ChengShengcheng Han

Abstract

The ERA (E. coli RAS-like protein)-related GTPase (ERG) is a nuclear-encoded GTPase with two conserved domains: a GTPase domain and a K Homology (KH) domain. ERG plays a vital role in early seed development in Antirrhinum majus. However, the mechanism that regulates seed development remains unclear. Blasting the genome sequence revealed two homologies of ERG, AtERG1, and AtERG2 in Arabidopsis. In this study, we found that AtERG2 is localized in the mitochondria and binds mitochondrial 18S RNA. Promoter and transcript analyses indicated that AtERG2 was mainly expressed in the leaf vein, trichome, and ovule. The T-DNA insertion lines of AtERG2 showed silique shortage, early seed abortion, and sporophytic maternal effects (SME), in which some seeds arrested in the zygotic stage at 1.5 days after pollination (DAP) and aborted at 2.0 DAP in aterg2-1 +/-. We further showed that the ovules of these arrested seeds presented unusual tissue degradation inside the embryo sacs. Reactive oxygen species (ROS) accumulated at 1.0 and 1.5 DAP in the arrested seeds, and the transcription of several ROS-responsive genes, WRKY40, ANAC017, and AOX1a, was up-regulated in the aterg2-1 +/- arrested seeds at 1.5 and 2.0 DAP, but not in wild-type (WT) a...Continue Reading

References

Mar 25, 1991·Nucleic Acids Research·K EdwardsC Thompson
Sep 1, 1989·Journal of Bacteriology·T InadaY Nakamura
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J AhnnM Inouye
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·C M Liu, D W Meinke
Mar 5, 1999·Science·M W GrayB F Lang
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Aug 28, 1999·Journal of Bacteriology·T I MeierG Zhao
Nov 24, 1999·Trends in Plant Science·J MarienfeldA Brennicke
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind
Oct 1, 1994·The Plant Cell·H. S. Yu, S. D. Russell
Mar 20, 2003·Plant Physiology·Vanessa VernoudErik Nielsen
Mar 17, 2007·Biochimica Et Biophysica Acta·Stéphane Pien, Ueli Grossniklaus
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chao TuXinhua Ji
Jun 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chao TuXinhua Ji
Sep 17, 2011·Biochimica Et Biophysica Acta·Takeshi Uchiumi, Dongchon Kang
Apr 19, 2015·BMC Biotechnology·Gerben P VosholCees A M J J van den Hondel
Jul 31, 2016·Seminars in Cell & Developmental Biology·Daisuke MaruyamaTetsuya Higashiyama
Sep 24, 2016·Trends in Biochemical Sciences·Shu-Ou Shan
Mar 9, 2017·Plant Physiology·Pritu PratibhaYelam Sreenivasulu

❮ Previous
Next ❯

Datasets Mentioned

BETA
ANAC017

Methods Mentioned

BETA
GTPase
PCR
transgenic
Protein Assay
electrophoresis
immunoprecipitation
RNA-IP

Software Mentioned

Data Processing System

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved