PMID: 2113911Jul 1, 1990Paper

The ermC leader peptide: amino acid alterations leading to differential efficiency of induction by macrolide-lincosamide-streptogramin B antibiotics

Journal of Bacteriology
M Mayford, B Weisblum

Abstract

The inducibility of ermC by erythromycin, megalomicin, and celesticetin was tested with both wild-type ermC and several regulatory mutants altered in the 19-amino-acid-residue leader peptide, MGIFSIFVISTVHYQP NKK. In the model test system that was used, the ErmC methylase was translationally fused to beta-galactosidase. Mutational alterations that mapped in the interval encoding Phe-4 through Ile-9 of the leader peptide not only affected induction by individual antibiotics, but did so differentially. The subset of mutations that affected inducibility by the two macrolides erythromycin and megalomicin overlapped and were distinct from the subset of mutations that affected induction by celesticetin. These studies provide a model system for experimentally varying the relative efficiencies with which different antibiotics induce the expression of ermC. The possibility that antibiotics with inducing activity interact directly with the nascent leader peptide was tested by using a chemically synthesized decapeptide, MGIFSIFVIS--, attached at its C-terminus to a solid-phase support. This peptide, however, failed to bind erythromycin in vitro.

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Citations

Nov 24, 1999·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·P ZhongL Katz
Apr 26, 2017·Antimicrobial Agents and Chemotherapy·Juliette R K WipfVincent Perreten
Jan 18, 2007·Archives of Pharmacal Research·Ae-Ran KwonEung-Chil Choi
Oct 28, 2008·The Journal of Antimicrobial Chemotherapy·Surbhi Malhotra-KumarHerman Goossens
Aug 17, 2016·Cold Spring Harbor Perspectives in Medicine·Corey FyfeJoyce Sutcliffe
Jan 18, 2006·The Journal of Biological Chemistry·Martin LovmarMåns Ehrenberg
Jan 5, 2002·Microbiology and Immunology·H J YunE C Choi
Jan 5, 2016·Nature Chemical Biology·Pulkit GuptaNora Vázquez-Laslop
Jan 28, 2009·Molecular Microbiology·Haripriya RamuNora Vazquez-Laslop
Feb 18, 2016·Nature Chemical Biology·Diego A Alonzo, T Martin Schmeing
Feb 2, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·G ClareboutR Leclercq
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nora Vázquez-LaslopAlexander S Mankin
Sep 29, 2005·Antimicrobial Agents and Chemotherapy·Jui-Chang TsaiLee-Jene Teng
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Yu-Hong MinEung-Chil Choi
Jan 16, 2007·Clinical Microbiology Reviews·Florence DepardieuPatrice Courvalin
Aug 15, 2002·Microbiology·Wolfgang LieblWolfgang Ludwig
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·T Tenson, A Mankin
Dec 19, 2007·Antimicrobial Agents and Chemotherapy·Marne BaileyAlexander S Mankin
Aug 21, 2020·Antimicrobial Agents and Chemotherapy·Vladimir VimbergGabriela Balikova Novotna
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Mar 1, 1997·Antimicrobial Agents and Chemotherapy·S Kamimiya, B Weisblum
Apr 29, 2008·Molecular Cell·Nora Vazquez-LaslopAlexander S Mankin

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