The Escherichia coli dnaA gene: four functional domains

Journal of Molecular Biology
M D Sutton, J M Kaguni

Abstract

The Escherichia coli DnaA protein is a sequence-specific DNA binding protein that promotes the initiation of replication of the bacterial chromosome, and of several plasmids including pSC101. Twenty-eight novel missense mutations of the E. coli dnaA gene were isolated by selecting for their inability to replicate a derivative of pSC101 when contained in a lambda vector. Characterization of these as well as seven novel nonsense mutations and one in-frame deletion mutation are described here. Results suggest that E. coli DnaA protein contains four functional domains. Mutations that affect residues in the P-loop or Walker A motif thought to be involved in ATP binding identify one domain. The second domain maps to a region near the C terminus and is involved in DNA binding. The function of the third domain that maps near the N terminus is unknown but may be involved in the ability of DnaA protein to oligomerize. Two alleles encoding different truncated gene products retained the ability to promote replication from the pSC101 origin but not oriC, identifying a fourth domain dispensable for replication of pSC101 but essential for replication from the bacterial chromosomal origin, oriC.

References

Jan 1, 1979·Methods in Enzymology·M KahnD R Helinski
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1977·Journal of Bacteriology·S N Cohen, A C Chang
Sep 9, 1977·Molecular & General Genetics : MGG·K Hasunuma, M Sekiguchi
Jun 1, 1978·Molecular & General Genetics : MGG·G Kellenberger-GujerL Caro
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1979·Molecular & General Genetics : MGG·J Felton, A Wright
Feb 1, 1992·Journal of Bacteriology·D P Biek, S N Cohen
Jul 1, 1992·Molecular & General Genetics : MGG·F G HansenT Atlung
May 1, 1992·Journal of Bacteriology·M J Calcutt, F J Schmidt
Jan 23, 1992·Nature·R M Story, T A Steitz
Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Jun 1, 1990·Microbiological Reviews·B J Bachmann
Oct 1, 1987·Molecular & General Genetics : MGG·Q P Wang, J M Kaguni
Sep 23, 1988·Cell·D Bramhill, A Kornberg
Jan 1, 1985·Molecular & General Genetics : MGG·T AtlungF G Hansen
Sep 1, 1986·Developmental Biology·L KroosD Kaiser
Oct 1, 1986·Journal of Bacteriology·B C KlineM S Shields
Oct 1, 1986·Molecular & General Genetics : MGG·C KüchererW Messer
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S H Wickner, D K Chattoraj
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E B Hansen, M B Yarmolinsky
Jul 19, 1974·Nature·M G RossmannK W Olsen
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P StougaardK Nordström
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Oct 20, 1995·Science·C M FraserJ C Venter
Jan 1, 1994·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·N OgasawaraH Yoshikawa

❮ Previous
Next ❯

Citations

Jul 3, 2010·The Journal of Biological Chemistry·Karl E DuderstadtJames M Berger
Apr 12, 2003·Nucleic Acids Research·Norie FujikawaShigeyuki Yokoyama
May 15, 2012·Nucleic Acids Research·Sylvain ZormanT R Strick
Sep 1, 2007·Science·Erin L Cunningham DueberJames M Berger
Jun 7, 2006·Annual Review of Microbiology·Jon M Kaguni
Jun 12, 2013·Annual Review of Biochemistry·Alessandro CostaJames M Berger
Feb 18, 2005·Journal of Biochemistry and Molecular Biology·Seok Hee Park
Jan 12, 2017·Genes·Katie H Jameson, Anthony J Wilkinson
Dec 14, 2004·Journal of Molecular Biology·M Asklund, T Atlung
Sep 12, 2001·The Journal of Biological Chemistry·K M Carr, J M Kaguni
Aug 8, 2007·Journal of Structural and Functional Genomics·Thomas J LoweryDavid E Wemmer
Jul 1, 2007·Biomolecular NMR Assignments·Yoshito AbeTadashi Ueda
May 10, 2005·The Journal of Biological Chemistry·Magdalena M FelczakJon M Kaguni
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Karl E Duderstadt, James M Berger
Sep 13, 2019·PLoS Genetics·Babatunde Ekundayo, Franziska Bleichert
Oct 9, 2002·Molecular Microbiology·Beatriz MaestroElena Fernández-Tresguerres
Sep 17, 2004·The Journal of Biological Chemistry·Magdalena M Felczak, Jon M Kaguni
May 20, 2005·The Journal of Biological Chemistry·Hironori KawakamiTsutomu Katayama
Feb 25, 2021·The Journal of Biological Chemistry·Ganesh K MauryaHari S Misra
Nov 26, 1999·Biochimie·W MesserJ Zakrzewska-Czerwinska
Nov 19, 2002·Biochemical and Biophysical Research Communications·Takayuki ObitaTaiji Imoto
Jun 25, 2015·Microbiology Spectrum·Igor KoniecznyKatarzyna Wegrzyn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.