The Escherichia coli heat labile toxin binds to Golgi membranes and alters Golgi and cell morphologies using ADP-ribosylation factor-dependent processes

The Journal of Biological Chemistry
X Zhu, Richard A Kahn

Abstract

The fate of the catalytic subunit of the Escherichia coli heat labile toxin (LTA(1)) was studied after expression in mammalian cells to assess the requirement for ADP-ribosylation factor (ARF) binding to localization and toxicity and ability to compete with endogenous ARF effectors. A progression in LTA(1) localization from cytosol to binding Golgi stacks to condensation of Golgi membranes was found to correlate with the time and level of LTA(1) expression. At the highest levels of LTA(1) expression the staining of LTA and both extrinsic and lumenal Golgi markers all became diffuse, in a fashion reminiscent of the actions of brefeldin A. Thus, LTA(1) binds to the Golgi and can alter its morphology in two distinct ways. However, point mutants of LTA(1) that are defective in the ability to bind activated ARF were also unable to bind Golgi membranes or modify Golgi morphology. Co-expression of mutants of ARF3 that regained binding to these same mutant LTA(1) proteins restored the localization and activities of the toxin. Thus, binding to ARF is required both for the localization of the toxin to the Golgi and for effects on Golgi membranes. A correlation was also seen between the ability of LTA mutants to bind ARF and the increase ...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Cassel, T Pfeuffer
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J G DonaldsonR D Klausner
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Nov 1, 1991·Cell·H R Pelham
Apr 1, 1995·Current Opinion in Structural Biology·E A Merritt, W G Hol
Jan 28, 1994·Science·S CockcroftJ J Hsuan
Feb 1, 1994·The Journal of Cell Biology·C J ZhangR A Kahn
Jan 1, 1995·Critical Reviews in Immunology·D P Snider
Dec 5, 1996·Nature·A PeyrocheC L Jackson
Mar 31, 1999·Immunology Today·N A WilliamsT O Nashar
Jul 9, 1999·Biochimica Et Biophysica Acta·W I LencerR K Holmes
May 2, 2000·The Journal of Biological Chemistry·X ZhuR A Kahn
Jun 20, 2000·The Journal of Biological Chemistry·M E MartinA Velasco

❮ Previous
Next ❯

Citations

Oct 13, 2006·Current Opinion in Gastroenterology·Lars Eckmann
Jun 19, 2004·Molecular Membrane Biology·Lolke De Haan, Timothy R Hirst
Feb 15, 2008·Journal of Molecular Biology·Ravi S AmpapathiGlen B Legge
Jan 1, 2006·EcoSal Plus·Michael G Jobling, Randall K Holmes
Aug 16, 2005·Science·Claire J O'NealWim G J Hol

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.