PMID: 9171392Jun 1, 1997Paper

The Escherichia coli histone-like protein HU affects DNA initiation, chromosome partitioning via MukB, and cell division via MinCDE

Journal of Bacteriology
A JaffeR D'Ari

Abstract

Escherichia coli hupA hupB double mutants, lacking both subunits (HU1 and HU2) of the histone-like protein HU, accumulate secondary mutations. In some genetic backgrounds, these include mutations in the minCDE operon, inactivating this system of septation control and resulting in the formation of minicells. In the course of the characterization of hupA hupB mutants, we observed that the simultaneous absence of the HU2 subunit and the MukB protein, implicated in chromosome partitioning, is lethal for the bacteria; the integrity of either HU or MukB thus seems to be essential for bacterial growth. The HU protein has been shown to be involved in DNA replication in vitro; we show here that its inactivation in the hupA hupB double mutant disturbs the synchrony of replication initiation in vivo, as evaluated by flow cytometry. Our results suggest that global nucleoid structure, determined in part by the histone-like protein HU, plays a role in DNA replication initiation, in proper chromosome partitioning directed by the MukFEB proteins, and in correct septum placement directed by the MinCDE proteins.

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Citations

Nov 28, 2000·Annual Review of Genetics·S Hiraga
Jul 27, 2002·Annual Review of Microbiology·Geoffrey C Draper, James W Gober
May 19, 2011·Helicobacter·Manuel ValenzuelaHéctor Toledo
Feb 24, 2015·G3 : Genes - Genomes - Genetics·Elisa Brambilla, Bianca Sclavi
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Beatrice RammPetra Schwille
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·W M HuangS X Yu
Jul 11, 2019·Journal of Biophotonics·Cynthia HansonElizabeth Vargis
Jun 13, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·John F Marko
Sep 24, 2020·Journal of Proteome Research·Valerie J Carabetta
Jun 14, 2000·Journal of Molecular Biology·A G PedersenD W Ussery
Aug 1, 2009·EcoSal Plus·Akira Ishihama
May 25, 2002·Molecular and Cellular Biology·Katherine MitsourasMichael Carey
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Nov 3, 2004·Plasmid·Santanu Dasgupta, Anders Løbner-Olesen
Jan 5, 2013·Insect Biochemistry and Molecular Biology·John F BeckmannAnn M Fallon

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