The Escherichia coli K-12 sheA gene encodes a 34-kDa secreted haemolysin

Molecular Microbiology
F J del CastilloI del Castillo

Abstract

Haemolytic toxins belong to one of several classes of virulence factors that contribute to bacterial pathogenicity. The non-pathogenic Escherichia coli K-12 laboratory strain was considered for years to be non-haemolytic. However, overproduction of several transcriptional regulators induced the appearance of a haemolytic activity that is absent under usual laboratory growth conditions. In this work, we have cloned and characterized an E. coli K-12 gene, sheA, whose overexpression results in a haemolytic phenotype. It maps to min 27 on the genetic map, and codes for a 34-kDa polypeptide with at least one putative transmembrane segment. This polypeptide, which has neither signal peptide nor other known secretory motifs, is secreted to the medium during the exponential growth phase. In vitro coupled transcription/translation assays, using a plasmid carrying only the sheA gene as template, resulted in the production of a polypeptide with haemolytic activity per se. Our results demonstrate that the sheA gene actually encodes the E. coli K-12 chromosomal haemolysin. The SheA haemolysin does not resemble other known cytolytic toxins, and it may represent the prototype of a novel family, as suggested by the presence of homologues in se...Continue Reading

Citations

Sep 19, 2009·The Journal of Microbiology·Jinghua CuiPhil Youl Ryu
Nov 4, 2010·The Journal of Microbiology·Lan Ji HuangPhil Youl Ryu
Nov 9, 2002·Biochimie·Felipe MorenoDaniel Bravo
May 5, 1999·Veterinary Microbiology·J ReingoldM D Lee
Jan 3, 2001·Molecular Microbiology·C A PritzlaffV Nizet
Jun 1, 2010·Journal of Bacteriology·Albrecht LudwigRoland Benz
Oct 29, 2000·Journal of Bacteriology·M WestermarkB E Uhlin
Sep 2, 2003·Journal of Bacteriology·Sun Nyunt WaiBernt Eric Uhlin
Aug 5, 2004·Journal of Bacteriology·Albrecht LudwigWerner Goebel
Mar 5, 2004·Journal of Bacteriology·Neil R WybornJeffrey Green
Jun 16, 2005·Journal of Clinical Microbiology·Monika KerényiTibor Pál
May 23, 2012·PLoS Pathogens·Guowei YangNicholas R Waterfield
Nov 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sudeshna KarSankar Adhya
Sep 5, 2013·Infection and Immunity·Nikki J WagnerVirginia L Miller
Sep 28, 2007·International Journal of Medical Microbiology : IJMM·Albrecht LudwigVolker Brade
Sep 26, 2007·Molecular Microbiology·James K LithgowJeffrey Green
Dec 3, 1998·Gastroenterology·A Darfeuille-MichaudJ F Colombel
Jun 17, 2014·International Journal of Medical Microbiology : IJMM·Martina BielaszewskaHelge Karch
May 31, 2008·Journal of Molecular Biology·Jae-Young KimMatthew P DeLisa
Feb 6, 2015·Nature Communications·Stephan BenkeBenjamin Schuler
Dec 4, 2001·FEMS Microbiology Letters·F J del CastilloI del Castillo

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