PMID: 9537393Apr 16, 1998Paper

The Escherichia coli mrsC gene is required for cell growth and mRNA decay

Journal of Bacteriology
L GrangerS R Kushner

Abstract

We have identified a gene in Escherichia coli that is required for both the normal decay of mRNA and RNA synthesis. Originally designated mrsC (mRNA stability), the mrsC505 mutation described here is, in fact, an allele of the hflB/ftsH locus (R.-F. Wang et al., J. Bacteriol. 180:1929-1938, 1998). Strains carrying the thermosensitive mrsC505 allele stopped growing soon after the temperature was shifted to 44 degrees C but remained viable for several hours. Net RNA synthesis stopped within 20 min after the shift, while DNA and protein synthesis continued for over 60 min. At 44 degrees C, the half-life of total pulse-labeled RNA rose from 2.9 min in a wild-type strain to 5.9 min in the mrsC505 single mutant. In an rne-1 mrsC505 double mutant, the average half-life was 19.8 min. Inactivating mrsC significantly increased the half-lives of the trxA, cat, secG, and kan mRNAs, particularly in an mrsC505 pnp-7 rnb-500 rne-1 multiple mutant. In addition, Northern analysis showed dramatic stabilizations of full-length mRNAs in a variety of mrsC505 multiple mutants at 44 degrees C. These results suggest that MrsC, directly or indirectly, controls endonucleolytic processing of mRNAs that may be independent of the RNase E-PNPase-RhlB multip...Continue Reading

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Citations

Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan A BernsteinStanley N Cohen
Aug 23, 2006·Infection and Immunity·M Regina SimionatoRichard J Lamont
Mar 17, 1999·FEMS Microbiology Reviews·W Schumann
Jun 17, 1999·FEMS Microbiology Reviews·A W Nicholson
Dec 14, 1999·Molecular Microbiology·B K Mohanty, S R Kushner
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Fei YuSteven R Rodermel
Jul 4, 2002·Folia Microbiologica·O Hlavácek, L Váchová
Jan 1, 2002·The Arabidopsis Book·Steven Rodermel
Jul 1, 2016·Journal of Integrative Plant Biology·Yanhui LinChuanqing Sun
Nov 26, 1999·Journal of Bacteriology·F NarberhausH Hennecke
Jul 20, 2000·Archives of Biochemistry and Biophysics·M M JayasekeraT P Holler
May 14, 1999·Current Opinion in Microbiology·S Gottesman
Apr 1, 2007·EcoSal Plus·Sidney R Kushner
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Sep 10, 2003·Biochemistry·Robert C BrucknerRoss L Stein
Oct 3, 2021·BMC Plant Biology·Qingfei WuXiao Ruan

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