The Escherichia coli multidrug transporter EmrE is a dimer in the detergent-solubilised state

Journal of Molecular Biology
P J G ButlerChristopher G Tate

Abstract

EmrE is a multidrug transporter that utilises the proton gradient across bacterial cell membranes to pump hydrophobic cationic toxins out of the cell. The structure of EmrE is very unusual, because it is an asymmetric homodimer containing eight alpha-helices, six of which form the substrate-binding chamber and translocation pathway. Despite this structural information, the precise oligomeric order of EmrE in both the detergent-solubilised state and in vivo is unclear, although it must contain an even number of subunits to satisfy substrate-binding data. We have studied the oligomeric state of EmrE, purified in a functional form in dodecylmaltoside, by high-resolution size-exclusion chromatography (hrSEC) and by analytical ultracentrifugation. The data from equilibrium analytical ultracentrifugation were analysed using a measured density increment for the EmrE-lipid-detergent complex, which showed that the purified EmrE was predominantly a dimer. This value was consistent with the apparent mass for the EmrE-lipid-detergent complex (137 kDa) determined by hrSEC. EmrE was purified under different conditions using minimal concentrations of dodecylmaltoside, which would have maintained the structure of any putative higher oligomeric...Continue Reading

References

Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Feb 1, 1997·Current Opinion in Biotechnology·W F Stafford
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S EskandariG A Zampighi
Mar 17, 1999·Biochimica Et Biophysica Acta·B TenchovG Rapp
Jan 11, 2001·Biophysical Chemistry·H Eisenberg
Mar 30, 2001·The Journal of Biological Chemistry·H YerushalmiS Schuldiner
Aug 28, 2002·Biochemistry·Francis I ValiyaveetilRoderick MacKinnon
Sep 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Misha SoskineShimon Schuldiner
Aug 30, 2003·Journal of Molecular Biology·Christopher G TateJoyce M Baldwin
Nov 25, 2003·The EMBO Journal·Iban Ubarretxena-BelandiaChristopher G Tate
Jan 1, 2004·The Journal of Biological Chemistry·Misha SoskineShimon Schuldiner
Feb 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yael ElbazShimon Schuldiner
Feb 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Che Ma, Geoffrey Chang

❮ Previous
Next ❯

Citations

Mar 1, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Daniel BastingClemens Glaubitz
Dec 20, 2011·Nature·Emma A MorrisonKatherine A Henzler-Wildman
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Gunnar von Heijne
Oct 19, 2004·The Journal of Biological Chemistry·Beata JastrzebskaKrzysztof Palczewski
Feb 20, 2009·The Journal of Biological Chemistry·Bradley E PoulsenCharles M Deber
Jan 28, 2009·Acta Crystallographica. Section D, Biological Crystallography·Vladimir M Korkhov, Christopher G Tate
Apr 25, 2012·Antimicrobial Agents and Chemotherapy·Bradley E Poulsen, Charles M Deber
Nov 27, 2008·BMC Biochemistry·Kalypso CharalambousPaula J Booth
May 21, 2009·Biological Chemistry·Ute A Hellmich, Clemens Glaubitz
Jan 27, 2007·Science·Mikaela RappGunnar von Heijne
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lisa BamberEdmund R S Kunji
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yen-Ju ChenGeoffrey Chang
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lisa BamberEdmund R S Kunji
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jim F WhiteReinhard Grisshammer
Jan 13, 2006·FEBS Letters·Owen Pornillos, Geoffrey Chang
Mar 20, 2009·Protein Expression and Purification·Tony WarneGebhard F X Schertler
Jan 27, 2009·Biochimica Et Biophysica Acta·Shimon Schuldiner
May 23, 2007·Biochemical and Biophysical Research Communications·Takashi KikukawaToshifumi Nara
Apr 25, 2007·Trends in Biochemical Sciences·Shimon Schuldiner
May 30, 2006·Protein Science : a Publication of the Protein Society·Andrei L LomizeHenry I Mosberg
Jun 6, 2006·Biochimica Et Biophysica Acta·Takashi KikukawaNaoki Kamo
Aug 27, 2014·Protein Science : a Publication of the Protein Society·Zhen GongPetra Fromme
Feb 15, 2005·Biochemical and Biophysical Research Communications·Haralambos KorresNaresh K Verma
Sep 29, 2006·Journal of Molecular Biology·Sarel J FleishmanNir Ben-Tal
Jul 11, 2006·Current Opinion in Structural Biology·Christopher G Tate
Aug 2, 2005·Biochemical and Biophysical Research Communications·Curtis W Sikora, Raymond J Turner
May 4, 2005·Protein Expression and Purification·Mark WestDavid L Christie
Oct 2, 2012·Journal of Molecular Biology·Rafael Núñez-RamírezArmando Albert
Dec 29, 2009·Biochimica Et Biophysica Acta·Denice C BayRaymond J Turner
Apr 1, 2014·Biochimica Et Biophysica Acta·Supratik DuttaKatherine A Henzler-Wildman
Jun 15, 2010·Structure·Vladimir M KorkhovChristopher G Tate
Feb 26, 2008·Journal of Molecular Biology·Vladimir M Korkhov, Christopher G Tate
Aug 25, 2009·Journal of Molecular Biology·David MillerPaula J Booth
May 24, 2011·Biochimica Et Biophysica Acta·Denice C Bay, Raymond J Turner
Oct 19, 2007·Biochimica Et Biophysica Acta·Denice C BayRaymond J Turner
Oct 27, 2007·Biochimica Et Biophysica Acta·Sébastien F Poget, Mark E Girvin
Nov 3, 2007·Biochimica Et Biophysica Acta·Vipin AgarwalBernd Reif
Apr 7, 2010·Biochimica Et Biophysica Acta·Edmund R S Kunji, Paul G Crichton
Oct 30, 2016·Journal of the American Chemical Society·Sangbae LeeNagarajan Vaidehi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.