The Escherichia coli O157 flagellar regulatory gene flhC and not the flagellin gene fliC impacts colonization of cattle.

Infection and Immunity
Heather S DobbinScott A Minnich

Abstract

A virulent European Escherichia coli O157:H- isolate is nonmotile due to a 12-bp deletion in the flagellar regulatory gene flhC. To investigate the contribution of flhC in the relationship between E. coli O157:H7 and cattle, we constructed a similar flhC regulatory mutant in the well-characterized strain ATCC 43894. There was no difference in the growth rate between the wild type and this regulatory mutant, but phenotypic arrays showed substrate utilization differences. Survival in the bovine gastrointestinal tract and colonization of the rectoanal junction mucosa were assessed. Mixtures of both strains were given orally or rectally to steers or administered into the rumen of cattle dually cannulated at the rumen and duodenum. One day post-oral dose, most rectal/fecal isolates (74%) were the regulatory mutant, but by 3 days post-oral dose and throughout the 42-day experiment, > or = 80% of the isolates were wild type. Among steers given a rectal application of both strains, wild-type isolates were the majority of isolates recovered on all days. The regulatory mutant survived better than the wild type in both the rumen and duodenum. To test the role of motility, a filament mutant (delta fliC) was constructed and similar cattle e...Continue Reading

References

Jan 1, 1973·Journal of Bacteriology·M Silverman, M Simon
Aug 1, 1997·FEMS Immunology and Medical Microbiology·I K WachsmuthM E Potter
Sep 3, 1998·Applied and Environmental Microbiology·I T KudvaC J Hovde
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·G M YoungV L Miller
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Apr 5, 2001·FEMS Microbiology Letters·B M PrüssP Matsumura
Jan 29, 2002·Epidemiology and Infection·T E BesserD D Hancock
Apr 5, 2002·Microbes and Infection·David G WhitePatrick F McDermott
Apr 27, 2002·Applied and Environmental Microbiology·Luke J GraukeCarolyn J Hovde
Jul 27, 2002·Journal of Bacteriology·Wen DengRobert D Perry
May 6, 2003·Annual Review of Microbiology·Robert M Macnab
Aug 6, 2004·Applied and Environmental Microbiology·Haiqing ShengCarolyn J Hovde
Oct 19, 2004·Trends in Microbiology·Hugo Cruz RamosJean-Claude Sirard
Jan 11, 2005·Applied and Environmental Microbiology·J Christopher LowDavid L Gally

❮ Previous
Next ❯

Citations

Apr 19, 2011·Applied and Environmental Microbiology·Preeti SuleBirgit M Prüss
Feb 2, 2010·The Veterinary Clinics of North America. Food Animal Practice·Rodney A Moxley, David R Smith
Feb 12, 2009·FEMS Microbiology Reviews·Roberto M La RagioneAndrew D Wales
Jan 16, 2015·PLoS Pathogens·Yannick RossezNicola J Holden
Oct 3, 2014·PLoS Genetics·Devon M FitzgeraldJoseph T Wade
Jan 1, 2013·Biology·Johanna Haiko, Benita Westerlund-Wikström
Jul 2, 2020·International Journal of Molecular Sciences·Elisa Andreozzi, Gaylen A Uhlich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.