The essential oil from Tagetes minuta L. modulates the binding of [3H]flunitrazepam to crude membranes from chick brain

Lipids
D A GarcíaI D Martijena

Abstract

The hypothesis that the essential oil from Tagetes minuta L. can interact with biological membranes was investigated by assessing its ability of perturbing the binding of a benzodiazepine [flunitrazepam (FNTZ)] to crude members from chick brains. The essential oil from T. minuta L. inhibited [3H]FNTZ specific binding to chick brain members. These values were obtained from the analysis of the saturation curve for the kinetic parameters: dissociation equilibrium constant (Kd) = 2.47 +/- 0.32 nM, maximal binding (Bmax) = 556 +/- 5 fmoles/mg protein, and Hill coefficient (n) = 1.00 +/- 0.07 in the absence, and Kd = 6.73 +/- 1.4 nM, Bmax = 583 +/- 69 fmoles/mg protein, and n = 1.02 +/- 0.08 in the presence of 29 microgram/mL of essential oil. The essential oil could self-aggregate with a critical micellar concentration (CMC) of 60 microgram/mL. The marked increase in [3H]FNTZ nonspecific binding starting at 60 micrograms of essence per mL was due to that phenomenon and revealed the ability of self-aggregated structures to interact with members. [3H]FNTZ specific binding decrement as a function of essence concentration cannot be ascribed merely to oil's micelles ability of trapping the lipophilic radioligand molecules, because the di...Continue Reading

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Citations

Oct 18, 2005·Journal of Chemical Ecology·María P Zunino, Julio A Zygadlo
Jul 21, 2004·Colloids and Surfaces. B, Biointerfaces·Mariela E SánchezMaría A Perillo
Jun 6, 2000·Journal of Ethnopharmacology·F A HamillD D Soejarto
Mar 28, 2006·Biophysical Chemistry·A del V TurinaM A Perillo
Feb 4, 2009·Journal of Pharmaceutical and Biomedical Analysis·Gabriela N ReinerDaniel A García
Oct 17, 2003·Biochimica Et Biophysica Acta·Anahí V TurinaMaría A Perillo

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