The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis

Microbiology
Anthony D Baughn, Michael H Malamy

Abstract

Haem is required for optimal growth of the bacterial anaerobe Bacteroides fragilis. Previous studies have shown that growth in the presence of haem is coincident with increased yields of ATP from glucose, expression of b-type cytochromes and expression of fumarate reductase activity. This paper describes the identification of the genes that encode the cytochrome, iron-sulfur cluster protein and flavoprotein of the B. fragilis fumarate reductase. These genes, frdC, frdA and frdB, respectively, are organized in an operon. Nonpolar, in-frame deletions of frdC and frdB were constructed in the B. fragilis chromosome. These mutant strains had no detectable fumarate reductase or succinate dehydrogenase activity. In addition, the frd mutant strains showed a threefold increase in generation time, relative to the wild-type strain. Growth of these mutant strains was fully restored to the wild-type rate by the introduction of a B. fragilis replicon containing the entire frd operon. Growth of the frd mutant strains was partially restored by supplementing the growth medium with succinate, indicating that the frd gene products function as a fumarate reductase. During growth on glucose, the frd mutant strains showed a threefold decrease in cel...Continue Reading

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Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anthony D Baughn, Michael H Malamy

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Citations

Dec 30, 2009·Antimicrobial Agents and Chemotherapy·Stuart DashperEric Reynolds
May 6, 2010·Future Microbiology·Vincent MeuricMartine Bonnaure-Mallet
Jul 20, 2012·Expert Review of Proteomics·Masae KuboniwaMurray Hackett
Jul 22, 2014·Molecular Oral Microbiology·C Anaya-BergmanJ P Lewis
Oct 25, 2011·Cell Host & Microbe·Michael A Fischbach, Justin L Sonnenburg
Dec 3, 2014·Anaerobe·Bikiran Pardesi, Geoffrey R Tompkins
Mar 13, 2019·Journal of Bacteriology·Adam MaynardBlanca Barquera
Nov 26, 2008·Journal of Bacteriology·Stuart G DashperEric C Reynolds
Mar 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zheng LuJames A Imlay
Sep 21, 2020·Microbial Pathogenesis·Mina YekaniMohammad Yousef Memar

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