The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton.

Plant Molecular Biology
Haihai WangTianzhen Zhang

Abstract

Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. Subsequently, the corresponding PEL genes were isolated from four different cotton species and characterized. In vitro enzyme assays indicated that GhPEL really exhibited cleavage-activity against de-esterified pectin. The temporal-spatial expression analyses revealed that the GhPEL gene was preferentially expressed in fibers at 10 days-post anthesis (DPA). Antisense GhPEL transgenic cotton plants were generated by Agrobacterium-mediated transformation. Six homozygous lines, each with one or two copies of the transgene inserted as determined by southern blot analysis of the NPTII gene, were selected for further functional analysis. The GhPEL expression during fiber elongation in these transgenic lines was significantly suppressed in various degrees. Furthermore, the reduction of GhPEL enzymatic activity by decreasing GhPEL transcripts severely affected the degradation of de-esterified pectin in primary ce...Continue Reading

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Citations

Jul 25, 2014·Journal of Experimental Botany·Fabien SénéchalJérôme Pelloux
Feb 12, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Caiping CaiWangzhen Guo
Mar 5, 2016·Frontiers in Plant Science·Ibrokhim Y AbdurakhmonovAlan E Pepper
Aug 19, 2014·The Plant Journal : for Cell and Molecular Biology·Nian LiuXianlong Zhang
Jun 16, 2016·Scientific Reports·Michael R Stiff, Candace H Haigler
Oct 15, 2013·Plant Biotechnology Journal·Deepti NigamSamir V Sawant
May 22, 2019·The Plant Journal : for Cell and Molecular Biology·Vikash KumarEwa J Mellerowicz

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